Recovery of Indigenous probiotic Lactobacillus plantarum Mut-7 on healthy Indonesian adults after consumption of fermented milk containing these bacteria

Probiotics are live microorganisms that confer health benefits on the host when administered in adequate amounts, such as to support the balance of gut microbiota. In this study, the selected indigenous probiotic strain, Lactobacillus plantarum Mut-7, was used as a starter culture to produce ferment...

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Published in:	Journal of food science and technology Vol. 58; no. 9; pp. 3525 - 3532
Main Authors:	Harahap, I. A., Mariyatun, M., Hasan, P. N., Pamungkaningtyas, F. H., Widada, J., Utami, T., Cahyanto, M. N., Juffrie, M., Dinoto, A., Nurfiani, S., Zulaichah, E., Sujaya, I. N., Rahayu, E. S.
Format:	Journal Article
Language:	English
Published:	New Delhi Springer India 01-09-2021 Springer Nature B.V
Subjects:	Adults Chemistry Chemistry and Materials Science Chemistry/Food Science Consumption Deoxyribonucleic acid DNA DNA sequencing E coli Feces Fermented milk products Food Science Gastrointestinal system Gastrointestinal tract Indigenous plants Ingestion Intestinal microflora Lactobacilli Lactobacillus plantarum Microbiota Microorganisms Milk Nutrition Original Original Article Polymerase chain reaction Probiotics Recovery rRNA 16S Starter cultures Indigenous probiotic Fermented milk Feces
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Summary:	Probiotics are live microorganisms that confer health benefits on the host when administered in adequate amounts, such as to support the balance of gut microbiota. In this study, the selected indigenous probiotic strain, Lactobacillus plantarum Mut-7, was used as a starter culture to produce fermented milk. A total of 28 healthy Indonesian youngsters and adults (male/female: 16/12; age 19.4–22.9 years old; normal BMI range 18.6–22.7 kg/m 2 ) were supplemented with the fermented milk. This study aimed to determine the recovery of L. plantarum Mut-7 through molecular analysis from the subjects feces after ingestion of 140 mL fermented milk containing 7.0 log 10 CFU/ml of L. plantarum Mut-7 for 15 days. Molecular detection was performed using the rep-PCR technique and sequencing of DNA 16S rRNA. Consumption of fermented milk containing L. plantarum Mut-7 enabled reduction of total E. coli and Coliform non- E. coli in several subjects. It was able to increase the total LAB and total L. plantarum in subjects’ feces. The number of L. plantarum and mesophilic LAB increased by 5.5 ± 1.6 log 10 CFU/g, 1.8 ± 0.8 log 10 . On the other side, thermophilic LAB increased by 2.8 ± 3.0 log 10 CFU/g in 23 out of 28 subjects. These findings proved that L. plantarum survived in the human gastrointestinal tract. Based on the molecular identification technique using rep-PCR technique and sequencing of gene 16S rRNA, two isolates had similarity to L. plantarum Mut-7 by a coefficient value of 100%.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-1155 0975-8402
DOI:	10.1007/s13197-021-05046-z