$Molecular detection of Papaya meleira virus in the latex of Carica papaya by RT-PCR$
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Molecular detection of Papaya meleira virus in the latex of Carica papaya by RT-PCR

A RT-PCR assay was developed for early and accurate detection of Papaya meleira virus (PMeV) in the latex from infected papayas. The meleira disease is characterized by an excessive exudation of more fluidic latex from fruits, leaves and stems. This latex oxidises and gives the fruit a “sticky” text...

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Bibliographic Details
Published in:	Journal of virological methods Vol. 146; no. 1; pp. 305 - 310
Main Authors:	Araújo, Marília Mendes Melo de, Tavares, Éder Torres, Silva, Felipe Rodrigues da, Marinho, Vera Lúcia de Almeida, Júnior, Manoel Teixeira Souza
Format:	Journal Article
Language:	English
Published:	London Elsevier B.V 01-12-2007 Amsterdam Elsevier New York, NY
Subjects:	Amino Acid Sequence Base Sequence Biological and medical sciences Carica - virology Carica papaya Diagnosis DNA Primers Fundamental and applied biological sciences. Psychology Latex Microbiology Molecular Sequence Data Papaya meleira disease Papaya “sticky” disease Plant Diseases - virology PMeV Random Amplified Polymorphic DNA Technique Reverse Transcriptase Polymerase Chain Reaction - methods RNA Viruses - chemistry RNA Viruses - genetics RNA Viruses - isolation & purification Techniques used in virology Viral Proteins - chemistry Viral Proteins - genetics Virology Virus Virus Papaya “sticky” disease Papaya meleira disease Diagnosis PMeV Microbiology Caricaceae Method Medicinal plant Virology Papaya sticky disease Dicotyledones Angiospermae Spermatophyta Fruit tree Carica papaya Detection Reverse transcription polymerase chain reaction
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Summary:	A RT-PCR assay was developed for early and accurate detection of Papaya meleira virus (PMeV) in the latex from infected papayas. The meleira disease is characterized by an excessive exudation of more fluidic latex from fruits, leaves and stems. This latex oxidises and gives the fruit a “sticky” texture. In the field, disease symptoms are seen almost exclusively on fruit. However, infected plants can be a source of virus for dissemination by insects. Primers specific for PMeV were designed based on nucleotide sequences of the viral dsRNA obtained using a RT-RAPD approach. When tested for RT-PCR amplification, one of these primers (C05-3′) amplified a 669-nucleotide fragment using dsRNA obtained from purified virus particles as a template. The translated sequence of this DNA fragment showed a certain degree of similarity to the amino acid sequence of RNA-dependent RNA polymerases from other dsRNA viruses. When used as the single primer in two RT-PCR kits available commercially, primer C05-3′ also amplified the DNA fragment from papaya latex of infected, but not from healthy plants. The RT-PCR-based method developed in this study could simplify early plant disease diagnosis, assist in monitoring the dissemination of the pathogen within and between fields, and assist in guiding plant disease management.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0166-0934 1879-0984
DOI:	10.1016/j.jviromet.2007.07.022