Effect of Surfactin Administration on Glucose and Lipid Metabolism in Mice Fed a High Fat Diet
Abstract only The high intake of high fat diet has been related with several changes on glucose and lipid metabolism and also on gut microbiota composition. Surfactin is an active compound produced by a wide variety of bacteria, fungi, and yeast that have important biological activities as anti‐tumo...
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| Published in: | The FASEB journal Vol. 31; no. S1 |
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| Main Authors: | , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
01-04-2017
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| Online Access: | Get full text |
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| Summary: | Abstract only
The high intake of high fat diet has been related with several changes on glucose and lipid metabolism and also on gut microbiota composition. Surfactin is an active compound produced by a wide variety of bacteria, fungi, and yeast that have important biological activities as anti‐tumour, anti‐microbial and anti‐inflammatory. Oral probiotics or their secreted factors are becoming the novel strategy for treatment of metabolism alterations. The aim of this study was to determine the influence of surfactin produced by
Bacillus subtilis (
ATCC 19659) on glucose and lipid metabolism in mice fed a high fat diet. Male mice (n=6/group) were fed with a control (C) or high fat (HF) diets and water
ad libitum
for 10 weeks. One group (HFS) was orally administrated with 5 mg/kg body weight of Surfactin once a week for 4 doses. Surfactin was isolated and purified from
Bacillus subtilis
culture. It was
lyophilized
and later dissolved in phosphate buffered saline (PBS) before the administration. Food (g) and water intake (ml) were measured daily. Body weight (g) was evaluated weekly. After 10 weeks, mice were euthanized and blood was collected. Using commercial kits the levels (mg/dl) of glucose (GLU), triglycerides (TG), total cholesterol (CHO), HDL and VLDL cholesterol were measured. Epididymal (EAT) and retroperitoneal (RAT) adipose tissue depots were collected and weighted (g). Results were expressed as mean ± SEM. Data were analyzed using T‐test. P<0.05 was considered significant for all data.
No difference was observed in body weight between the groups however HF and HFS groups eat and drink less than C. An increase of ~47% at EAT was observed in HL (2.69±0.31) vs C (1.83±0.18). There was no difference at RAT in any group. There was an increase of ~27% in HF (362.7±11) vs C (284.9±30) and a decrease of ~20% in HFS (289.3±34) vs HF (362.9±11) at GLU. An increase of ~47% at CHO in HF (155.5±10.9) vs C (105.6±3.4) and also in HDL (~59%) HF (112.4±6.5) vs C (70.4±3.9) was observed. TAG was decreased ~32% in HF (109.8±2.9) vs C (162.9±19.1) and also in HFS (~30%) (76.4±11) vs HL (109.8±2.9). Same pattern of results were observed in VLDL. In conclusion, we observed that HF diet intake was able to disrupt glucose and lipid metabolism and surfactin seems to be efficient in the control of glycemia and tryglyceredemia. We hipothesize that these effects achieved are due surfactin action on anti‐inflammatory cytokines production and/or changes in gut microbiota composition.
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| ISSN: | 0892-6638 1530-6860 |
| DOI: | 10.1096/fasebj.31.1_supplement.944.14 |