Search Results - "Marcos, João C"

Simplified 2,4-dinitrophenylhydrazine spectrophotometric assay for quantification of carbonyls in oxidized proteins by Mesquita, Cristina S., Oliveira, Raquel, Bento, Fátima, Geraldo, Dulce, Rodrigues, João V., Marcos, João C.

“…This work proposes a modification of the 2,4-dinitrophenylhydrazine (DNPH) spectrophotometric assay commonly used to evaluate the concentration of carbonyl…”
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Protein folding and quinary interactions: creating cellular organisation through functional disorder by Ribeiro, Sara, Ebbinghaus, Simon, Marcos, João C.

“…The marginal stability of globular proteins in the cell is determined by the balance between excluded volume effect and soft interactions. Quinary interactions…”
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Hofmeister effects on protein stability are dependent on the nature of the unfolded state by Ribeiro, Sara S, Castro, Tarsila G, Gomes, Cláudio M, Marcos, João C

“…The interpretation of a salt's effect on protein stability traditionally discriminates low concentration regimes (<0.3 M), dominated by electrostatic forces,…”
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The synergic effect of water and biomolecules in intracellular phase separation by Ribeiro, Sara S., Samanta, Nirnay, Ebbinghaus, Simon, Marcos, João C.

“…Phase separation has long been observed within aqueous mixtures of two or more different compounds, such as proteins, salts, polysaccharides and synthetic…”
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Non-Canonical Amino Acids as Building Blocks for Peptidomimetics: Structure, Function, and Applications by Castro, Tarsila G, Melle-Franco, Manuel, Sousa, Cristina E A, Cavaco-Paulo, Artur, Marcos, João C

“…This review provides a fresh overview of non-canonical amino acids and their applications in the design of peptidomimetics. Non-canonical amino acids appear…”
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Protein quantification in the presence of poly(ethylene glycol) and dextran using the Bradford method by Barbosa, Helder, Slater, Nigel K.H., Marcos, João C.

“…Some experimental methodologies require the quantification of protein in the presence of polymers like poly(ethylene glycol) (PEG) and dextran (DEX). In the…”
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Initial Screening of Poly(ethylene glycol) Amino Ligands for Affinity Purification of Plasmid DNA in Aqueous Two-Phase Systems by da Silva, Nuno R., Jorge, Paula, Martins, José A., Teixeira, José A., Marcos, João C.

“…Gene therapy and DNA vaccination are among the most expected biotechnological and medical advances for the coming years. However, the lack of cost-effective…”
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Purification of plasmid DNA vectors by aqueous two-phase extraction and hydrophobic interaction chromatography by Trindade, Inês P., Diogo, Maria M., Prazeres, Duarte M.F., Marcos, João C.

“…The current study explores the possibility of using a polyethyleneglycol(PEG)–ammonium sulphate aqueous two-phase system (ATPS) as an early step in a process…”
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Preparation of plasmid DNA polyplexes from alkaline lysates by a two-step aqueous two-phase extraction process by Duarte, Sónia P., Fortes, António G., Prazeres, Duarte M.F., Marcos, João C.

“…This work studied the possibility of using polyethyleimine (PEI) as an affinity ligand for the purification of plasmid DNA (pDNA) from alkaline lysates using…”
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Assessing the Impact of Nontraditional Ocean Observations for Prediction of the East Australian Current by Siripatana, Adil, Kerry, Colette, Roughan, Moninya, Souza, João Marcos A. C., Keating, Shane

“…Accurate forecasting of ocean currents in dynamic regions remains a critical challenge due to the sparsity of observations in global ocean observing networks…”
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Paravertebral extramedullary haemopoiesis in a patient with pachydermoperiostosis by Souto Filho, João T. D., Morais, Rodrigo A., Ribeiro, Helena A. A., Ribeiro, João Marcos M. C., Ferreira Júnior, Wellington D. S., Figueiredo, Luiz C. S.

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Comparison of 4-dimensional variational and ensemble optimal interpolation data assimilation systems using a Regional Ocean Modeling System (v3.4) configuration of the eddy-dominated East Australian Current system by Kerry, Colette Gabrielle, Roughan, Moninya, Keating, Shane, Gwyther, David, Brassington, Gary, Siripatana, Adil, Souza, Joao Marcos A. C

“…Ocean models must be regularly updated through the assimilation of observations (data assimilation) in order to correctly represent the timing and locations of…”
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On the Effect of Peak Current-Front Time Correlation Expressions for the Assessment of the Lightning Performance of Transmission Lines by Silveira, Fernando H., Bichara, Pedro M., Oliveira, Joao Marcos C., Visacro, Silverio

“…This work evaluates the influence of considering correlation expressions between peak current and current front time of negative first return strokes on the…”
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Integration of plasmid DNA purification and molecular therapy agent preparation using aqueous two-phase systems by Duarte, Sónia, Fortes, António G., Prazeres, Duarte. M., Marcos, João C.

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