Differential gene expression profile of multinodular goiter

Differential gene expression profile of multinodular goiter

The goiter, a neglected heterogeneous molecular disease, remains a major indication for thyroidectomies in its endemic regions. This study analyzed differential gene expression in surgical specimens diagnosed with multi nodular and compared the data to that of thyroid tissue without multinodular goi...

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Bibliographic Details
Published in:PloS one Vol. 17; no. 5; p. e0268354
Main Authors: de Figueiredo, Wenberger Lanza Daniel, Lopes, Eraldo Ferreira, Jezini, Deborah Laredo, Marçal, Lorena Naciff, de Assunção, Enedina Nogueira, Ribeiro Rodrigues, Paulo Rodrigo, José da Mota, Adolfo, Carvalho, Diego Monteiro de, Filho, Spartaco Astolfi, Lopes Botelho, João Bosco
Format: Journal Article
Language:English
Published: United States Public Library of Science 20-05-2022
Public Library of Science (PLoS)
Subjects:
Analysis
Apoptosis
Biology and Life Sciences
Breast cancer
Cell cycle
Diagnosis
Disease
Gene expression
Gene sequencing
Genetic aspects
Goiter
Health aspects
Hypoxia
Medical practices
Medicine and Health Sciences
Mutation
Proteins
Research and analysis methods
Ribonucleic acid
RNA
Thyroid
Thyroid cancer
Thyroidectomy
Tissues
Transcriptomes
Tumors
Brazil
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Summary:The goiter, a neglected heterogeneous molecular disease, remains a major indication for thyroidectomies in its endemic regions. This study analyzed differential gene expression in surgical specimens diagnosed with multi nodular and compared the data to that of thyroid tissue without multinodular goiter from patients undergoing thyroidectomy in Manaus-AM, Brazil using RNA-seq technology. The transcriptome information of the surgical specimen fragments with and without multinodular goiter was accessed by Illumina HiSeq 2000 New Generation Sequencing (NGS) using the RNA-seq NEBNext® Ultra™ RNA Library Prep Kit for Illumina®-#E7530L protocol and differential gene expression analysis. Differences were found between the gene expression profiles of the diseased tissues and those of the healthy control tissues; at least 70 genes were differentially expressed. The HOTS gene was expressed only in multinodular goiter tissues (p < 0.05). These results demonstrate that the gene expression profile of multinodular goiter is pro-tumoral and that HOTS can play a central role in multinodular goiter development.
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Competing Interests: The authors have declared that no competing interests exist.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0268354