Synergy at work: linking the metabolism of two lactic acid bacteria to achieve superior production of 2-butanol

Synergy at work: linking the metabolism of two lactic acid bacteria to achieve superior production of 2-butanol

The secondary alcohol 2-butanol has many important applications, e.g., as a solvent. Industrially, it is usually made by sulfuric acid-catalyzed hydration of butenes. Microbial production of 2-butanol has also been attempted, however, with little success as witnessed by the low titers and yields rep...

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Published in:Biotechnology for biofuels Vol. 13; no. 1; p. 45
Main Authors: Mar, Mette J, Andersen, Joakim M, Kandasamy, Vijayalakshmi, Liu, Jianming, Solem, Christian, Jensen, Peter R
Format: Journal Article
Language:English
Published: England BioMed Central Ltd 11-03-2020
BioMed Central
BMC
Subjects:
2-Butanol
Alcohol
Bacteria
Butanol
Chemical properties
Co-cultivation
Control
Dairy industry
Dehydrogenases
Diol dehydratase
Enzymes
Ethanol
Fermentation
Genetic engineering
Glycerol
Lactic acid
Lactic acid bacteria
Lactobacillus brevis
Lactococcus
Lactococcus lactis
Metabolic engineering
Metabolism
Methods
Microbiological synthesis
Microorganisms
Production processes
Denmark
Diol dehydratase
Lactococcus lactis
Co-cultivation
2-Butanol
Lactobacillus brevis
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Summary:The secondary alcohol 2-butanol has many important applications, e.g., as a solvent. Industrially, it is usually made by sulfuric acid-catalyzed hydration of butenes. Microbial production of 2-butanol has also been attempted, however, with little success as witnessed by the low titers and yields reported. Two important reasons for this, are the growth-hampering effect of 2-butanol on microorganisms, and challenges associated with one of the key enzymes involved in its production, namely diol dehydratase. We attempt to link the metabolism of an engineered strain, which possesses all enzyme activities required for fermentative production of 2-butanol from glucose, except for diol dehydratase, which acts on -2,3-butanediol (mBDO), with that of a strain which expresses a functional dehydratase natively. We demonstrate growth-coupled production of 2-butanol by the engineered strain, when co-cultured with . After fine-tuning the co-culture setup, a titer of 80 mM (5.9 g/L) 2-butanol, with a high yield of 0.58 mol/mol is achieved. Here, we demonstrate that it is possible to link the metabolism of two bacteria to achieve redox-balanced production of 2-butanol. Using a simple co-cultivation setup, we achieved the highest titer and yield from glucose in a single fermentation step ever reported. The data highlight the potential that lies in harnessing microbial synergies for producing valuable compounds.
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ISSN:1754-6834
1754-6834
DOI:10.1186/s13068-020-01689-w