Flow cytometry and biochemical analysis of DNA degradation characteristic of two types of cell death

Flow cytometry and biochemical analysis of DNA degradation characteristic of two types of cell death

By flow cytometry it has been shown that in rat thymocytes dying upon γ-irradiation the reduction in DNA content per cell occurs well before the increase in outer membrane permeability. In contrast, in irradiated Burkitt's lymphoma cells the disturbance of plasma membrane permeability precedes...

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Bibliographic Details
Published in:FEBS letters Vol. 194; no. 2; pp. 351 - 354
Main Authors: Afanas'ev, Vladimir N., Korol', Boris A., Mantsygin, Yurii A., Nelipovich, Piotr A., Pechatnikov, Vladimir A., Umansky, Samuil R.
Format: Journal Article
Language:English
Published: Elsevier B.V 06-01-1986
Subjects:
Cell death
DNA degradation
Flow cytometry
Membrane permeability
Flow cytometry
DNA degradation
Cell death
Membrane permeability
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Summary:By flow cytometry it has been shown that in rat thymocytes dying upon γ-irradiation the reduction in DNA content per cell occurs well before the increase in outer membrane permeability. In contrast, in irradiated Burkitt's lymphoma cells the disturbance of plasma membrane permeability precedes the decrease of DNA content. Genome degradation in dying thymocytes of irradiated or hydrocortisone-treated rats is accounted for by internucleosomal DNA fragmentation. Postirradiation DNA cleavage in the 3 investigated cell cultures is disorderly and is probably caused by the activation of hydrolases in dead cells.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(86)80115-6