Peptosome: A New Efficient Transfection Tool as an Alternative to Liposome

Peptosome: A New Efficient Transfection Tool as an Alternative to Liposome

Gene therapy is one of the most promising techniques for treating genetic diseases and cancer. The current most important problem in gene therapy is gene delivery. Viral and non-viral vectors like liposomes, used for gene delivery, have many limitations. We have developed new hybrid peptides by comb...

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Bibliographic Details
Published in:International journal of molecular sciences Vol. 25; no. 13; p. 6918
Main Authors: Manteghi, Maliheh, Can, Ozge, Kocagoz, Tanil
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 24-06-2024
Subjects:
Amino acids
Antigens
Cancer
cell-penetrating peptide
Cell-Penetrating Peptides - chemistry
Cells
CRISPR
Cytotoxicity
Efficiency
Enzymes
gene delivery
Gene expression
Gene therapy
Gene Transfer Techniques
Genetic disorders
Genetic engineering
Genetic Therapy - methods
Genomes
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
HeLa Cells
histone
Histones - chemistry
Histones - genetics
Histones - metabolism
Humans
Lipids
Liposomes - chemistry
mRNA vaccines
Nanoparticles
Peptides
Plasmids - genetics
Polymers
Transfection - methods
Vectors (Biology)
gene delivery
gene therapy
histone
cell-penetrating peptide
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Summary:Gene therapy is one of the most promising techniques for treating genetic diseases and cancer. The current most important problem in gene therapy is gene delivery. Viral and non-viral vectors like liposomes, used for gene delivery, have many limitations. We have developed new hybrid peptides by combining cell-penetrating peptides (CPPs) with the DNA-binding domain of the human histone H4 protein. These small peptides bind to DNA molecules through their histone domain, leaving the CPP part free and available for binding and penetration into cells, forming complexes that we named "peptosomes". We evaluated the transfection efficiency of several hybrid peptides by delivering a plasmid carrying the green fluorescent protein gene and following its expression by fluorescent microscopy. Among several hybrid peptides, TM3 achieved a gene delivery efficiency of 76%, compared to 52% for Lipofectamine 2000. TM3 peptosomes may become important gene delivery tools with several advantages over current gene delivery agents.
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25136918