Search Results - "Mann, Matthias"

Functional and quantitative proteomics using SILAC by Mann, Matthias

“…Researchers in many biological areas now routinely characterize proteins by mass spectrometry. Among the many formats for quantitative proteomics,…”
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The ever expanding scope of electrospray mass spectrometry—a 30 year journey by Mann, Matthias

“…John Fenn’s electrospray mass spectrometry (ESMS) was awarded the chemistry Nobel Prize in 2002 and is now the basis of the entire field of MS-based…”
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Mass-spectrometric exploration of proteome structure and function by Aebersold, Ruedi, Mann, Matthias

“…Numerous biological processes are concurrently and coordinately active in every living cell. Each of them encompasses synthetic, catalytic and regulatory…”
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1D and 2D annotation enrichment: a statistical method integrating quantitative proteomics with complementary high-throughput data by Cox, Juergen, Mann, Matthias

“…Quantitative proteomics now provides abundance ratios for thousands of proteins upon perturbations. These need to be functionally interpreted and correlated to…”
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Status of Large-scale Analysis of Post-translational Modifications by Mass Spectrometry by Olsen, Jesper V., Mann, Matthias

“…Cellular function can be controlled through the gene expression program, but often protein post-translational modifications (PTMs) provide a more precise and…”
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A Primer on Concepts and Applications of Proteomics in Neuroscience by Hosp, Fabian, Mann, Matthias

“…The enormous complexity of the central nervous system has impeded its systemic exploration for decades but powerful “omic” technologies are now pushing forward…”
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MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification by Cox, Jürgen, Mann, Matthias

“…Cox and Mann describe MaxQuant, a suite of algorithms for the analysis of high-resolution mass spectrometry data. The approach achieves substantial…”
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Revisiting biomarker discovery by plasma proteomics by Geyer, Philipp E, Holdt, Lesca M, Teupser, Daniel, Mann, Matthias

“…Clinical analysis of blood is the most widespread diagnostic procedure in medicine, and blood biomarkers are used to categorize patients and to support…”
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The Perseus computational platform for comprehensive analysis of (prote)omics data by Tyanova, Stefka, Temu, Tikira, Sinitcyn, Pavel, Carlson, Arthur, Hein, Marco Y, Geiger, Tamar, Mann, Matthias, Cox, Jürgen

“…Perseus is a comprehensive, user-friendly software platform for the biological analysis of quantitative proteomics data. It is intended to help biologists with…”
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Minimal, encapsulated proteomic-sample processing applied to copy-number estimation in eukaryotic cells by Kulak, Nils A, Pichler, Garwin, Paron, Igor, Nagaraj, Nagarjuna, Mann, Matthias

“…A streamlined, robust sample-preparation method for mass spectrometry–based proteome analysis is reported. All sample preparation steps are carried out in a…”
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More than 100,000 Detectable Peptide Species Elute in Single Shotgun Proteomics Runs but the Majority is Inaccessible to Data-Dependent LC−MS/MS by Michalski, Annette, Cox, Juergen, Mann, Matthias

“…Shotgun proteomics entails the identification of as many peptides as possible from complex mixtures. Here we investigate how many peptides are detectable by…”
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Copy Number Analysis of the Murine Platelet Proteome Spanning the Complete Abundance Range by Zeiler, Marlis, Moser, Markus, Mann, Matthias

“…Knowledge of the identity and quantity of expressed proteins of a cell type is a prerequisite for a complete understanding of its molecular functions…”
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Quantitative Analysis of the Intra- and Inter-Individual Variability of the Normal Urinary Proteome by Nagaraj, Nagarjuna, Mann, Matthias

“…Urine is a readily and noninvasively obtainable body fluid. Mass spectrometry (MS)-based proteomics has shown that urine contains thousands of proteins. Urine…”
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Comparative Proteomic Analysis of Eleven Common Cell Lines Reveals Ubiquitous but Varying Expression of Most Proteins by Geiger, Tamar, Wehner, Anja, Schaab, Christoph, Cox, Juergen, Mann, Matthias

“…Deep proteomic analysis of mammalian cell lines would yield an inventory of the building blocks of the most commonly used systems in biological research. Mass…”
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Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology by Robles, Maria S., Humphrey, Sean J., Mann, Matthias

“…Circadian clocks are self-sustainable endogenous oscillators, present in virtually every cell, driving daily cycles of metabolism and physiology. The molecular…”
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Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips by Rappsilber, Juri, Mann, Matthias, Ishihama, Yasushi

“…Mass spectrometry (MS)-based proteomics measures peptides derived from proteins by proteolytic cleavage. Before performing the analysis by matrix-assisted…”
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Protein Phosphorylation: A Major Switch Mechanism for Metabolic Regulation by Humphrey, Sean J, James, David E, Mann, Matthias

“…Metabolism research is undergoing a renaissance because many diseases are increasingly recognized as being characterized by perturbations in intracellular…”
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High-throughput phosphoproteomics reveals in vivo insulin signaling dynamics by Humphrey, Sean J, Azimifar, S Babak, Mann, Matthias

“…Phosphoproteomes are rapidly measured in parallel to track the dynamics of insulin signaling in the liver. Mass spectrometry has enabled the study of cellular…”
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Accurate Proteome-wide Label-free Quantification by Delayed Normalization and Maximal Peptide Ratio Extraction, Termed MaxLFQ by Cox, Jürgen, Hein, Marco Y., Luber, Christian A., Paron, Igor, Nagaraj, Nagarjuna, Mann, Matthias

“…Protein quantification without isotopic labels has been a long-standing interest in the proteomics field. However, accurate and robust proteome-wide…”
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Proteomics to study DNA-bound and chromatin-associated gene regulatory complexes by Wierer, Michael, Mann, Matthias

“…High-resolution mass spectrometry (MS)-based proteomics is a powerful method for the identification of soluble protein complexes and large-scale affinity…”
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