Identification of Optimal Tissue-Marking Dye Color for Pathological Evaluation in Fluorescence Imaging Using IRDye800CW

Identification of Optimal Tissue-Marking Dye Color for Pathological Evaluation in Fluorescence Imaging Using IRDye800CW

Purpose Fluorescence-guided surgery using a tumor-specific antibody-dye conjugate is useful in various cancer types. Fluorescence imaging is a valuable tool both intraoperatively and postoperatively for ex vivo imaging. The color of inks used for tumor specimens during ex vivo specimen processing in...

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Published in:Molecular imaging and biology Vol. 26; no. 1; pp. 162 - 172
Main Authors: Kondo, Takahito, Nishio, Naoki, Park, Jason S., Mani, Lucas D., Naveed, Abdullah, Tanaka, Hidenori, Lewis, James S., Rosenthal, Eben L., Hom, Marisa E.
Format: Journal Article
Language:English
Published: Cham Springer International Publishing 01-02-2024
Springer Nature B.V
Subjects:
Animals
Antibodies
Calcium Compounds
Cancer
Citrus fruits
Color
Dyes
Emission analysis
Evaluation
Fluorescence
Fluorescent Dyes
Fluorescent indicators
Fruits
Head and neck carcinoma
Head and Neck Neoplasms
Heat detection
Humans
Imaging
Inks
Marking
Medical imaging
Medicine
Medicine & Public Health
Mice
Optical Imaging - methods
Oxides
Radiology
Research Article
Squamous cell carcinoma
Squamous Cell Carcinoma of Head and Neck
Tumors
Xenotransplantation
Tissue-marking dye
Fluorescence imaging
Surgical margin assessment
IRDye800CW
Fluorescence-guided surgery
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Summary:Purpose Fluorescence-guided surgery using a tumor-specific antibody-dye conjugate is useful in various cancer types. Fluorescence imaging is a valuable tool both intraoperatively and postoperatively for ex vivo imaging. The color of inks used for tumor specimens during ex vivo specimen processing in pathology is an important consideration for fluorescence imaging since the absorption/emission of the dyes may interfere with the fluorescent dye. This study assesses suitable ink colors for use specifically with IRDye800CW fluorescence imaging. Procedures Eight tissue-marking inks or dyes (TMDs) commonly used for pathological evaluation were assessed. Agarose tissue-mimicking phantoms containing Panitumumab-IRDye800CW were used as an initial model. Mean fluorescence intensity was measured at 800 nm using both Pearl Trilogy as a closed-field fluorescence imaging system and pde-neo II as an open-field fluorescence imaging system before and after TMD application. An in vivo mouse xenograft model using the human head and neck squamous cell carcinoma FaDu cell line was then used in conjunction with TMDs. Results The retained IRDye800CW fluorescence on Pearl Trilogy was as follows: yellow at 91.0 ± 4.5%, red at 90.6 ± 2.7%, orange at 88.2 ± 2.2%, violet at 56.6 ± 1.1%, lime at 40.9 ± 1.8%, green at 19.3 ± 2.8%, black at 13.3 ± 0.6%, and blue at 8.1 ± 0.2%. The retained IRDye800CW fluorescence on pde-neo II was as follows: yellow at 86.5 ± 6.4%, red at 77.0 ± 6.2%, orange at 76.9 ± 2.8%, lime at 72.5 ± 9.5%, violet at 59.7 ± 0.4%, green at 30.1 ± 6.9%, black at 17.0 ± 2.7%, and blue at 6.7 ± 1.7%. The retained IRDye800CW fluorescence in yellow and blue TMDs was 42.1 ± 14.9% and 0.2 ± 0.2%, respectively in the mouse experiment ( p  = 0.039). Conclusion Yellow, red, and orange TMDs should be used, and blue and black TMDs should be avoided for evaluating tumor specimens through fluorescence imaging using IRDye800CW.
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content type line 23
ISSN:1536-1632
1860-2002
DOI:10.1007/s11307-023-01882-x