Tomato yellow spot virus, a tomato-infecting begomovirus from Brazil with a closer relationship to viruses from Sida sp., forms pseudorecombinants with begomoviruses from tomato but not from Sida

Tomato yellow spot virus, a tomato-infecting begomovirus from Brazil with a closer relationship to viruses from Sida sp., forms pseudorecombinants with begomoviruses from tomato but not from Sida

Geminiviruses are characterized by a circular, single-stranded DNA genome and twinned icosahedral particles. Begomoviruses (whitefly-transmitted geminiviruses) are a major constraint to crop production worldwide. In Brazil, tomato-infecting begomoviruses emerged as serious pathogens over the last 10...

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Bibliographic Details
Published in:Journal of general virology Vol. 87; no. 12; pp. 3687 - 3696
Main Authors: Andrade, E.C, Manhani, G.G, Alfenas, P.F, Calegario, R.F, Fontes, E.P.B, Zerbini, F.M
Format: Journal Article
Language:English
Published: Reading Soc General Microbiol 01-12-2006
Society for General Microbiology
Subjects:
Amino Acid Sequence
Base Sequence
Begomovirus
Begomovirus - genetics
Begomovirus - isolation & purification
Begomovirus - physiology
Biological and medical sciences
Brazil
DNA, Viral - analysis
DNA, Viral - genetics
Fundamental and applied biological sciences. Psychology
Geminiviridae
Lycopersicon esculentum
Malvaceae - virology
Microbial Viability
Microbiology
Miscellaneous
Molecular Sequence Data
Nicotiana - virology
Nicotiana benthamiana
Phylogeny
Plant Diseases - virology
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Recombination, Genetic
Replication Origin - genetics
Sequence Homology
Sida
Solanum lycopersicum - virology
Tomato golden mosaic virus
tomatoes
Virology
Brazil
South America
America
Virus
Plant pathogen
Vegetable crop
Microbiology
Dicotyledones
Angiospermae
Lycopersicon esculentum
Geminiviridae
Spermatophyta
Solanaceae
Begomovirus
Virology
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Description
Summary:Geminiviruses are characterized by a circular, single-stranded DNA genome and twinned icosahedral particles. Begomoviruses (whitefly-transmitted geminiviruses) are a major constraint to crop production worldwide. In Brazil, tomato-infecting begomoviruses emerged as serious pathogens over the last 10 years, due to the introduction of a new biotype of the insect vector. Tomato yellow spot virus (ToYSV) is a newly described begomovirus originally isolated from tomato, but phylogenetically closer to viruses from Sida sp. A study was performed to determine the viability of pseudorecombinants formed between the DNA components of ToYSV and other weed- and tomato-infecting begomoviruses from Brazil. Despite its closer relationship to weed-infecting viruses, ToYSV was only capable of forming viable pseudorecombinants with tomato viruses. An infectious pseudorecombinant formed between ToYSV DNA-A and tomato crinkle leaf yellows virus (TCrLYV) DNA-B induced severe symptoms in Nicotiana benthamiana. This was attributed, at least in part, to the fact that the origins of replication of both components had identical Rep-binding sequences. However, this was not the case for another infectious pseudorecombinant formed between tomato golden mosaic virus (TGMV) DNA-A and ToYSV DNA-B, which have different Rep-binding sequences. These results reinforce the notion that pseudorecombinant formation cannot be explained solely on the basis of phylogenetic relationships and conserved iteron sequences, and suggest that the TGMV Rep protein may be more versatile in terms of recognizing heterologous DNA components than that of ToYSV.
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ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.82279-0