Increased T helper 1 cytokine responses by circulating T cells are present in women with recurrent pregnancy losses and in infertile women with multiple implantation failures after IVF

BACKGROUND: We aimed to study T‐helper 1 (Th1) and Th2 intracellular cytokine expression in peripheral blood lymphocytes of women with recurrent spontaneous abortions (RSA) or infertility with multiple implantation failures after IVF cycles. METHODS: Twenty‐six women with three or more RSA and 23 wi...

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Bibliographic Details
Published in:	Human reproduction (Oxford) Vol. 18; no. 4; pp. 767 - 773
Main Authors:	Kwak‐Kim, J.Y.H., Chung‐Bang, H.S., Ng, S.C., Ntrivalas, E.I., Mangubat, C.P., Beaman, K.D., Beer, A.E., Gilman‐Sachs, A.
Format:	Journal Article
Language:	English
Published:	Oxford Oxford University Press 01-04-2003 Oxford Publishing Limited (England)
Subjects:	Abortion, Habitual - blood Adult Biological and medical sciences Blood Cells - pathology Case-Control Studies CD3 Complex - analysis CD8 Antigens - analysis Cytokines - blood Diseases of mother, fetus and pregnancy Embryo Implantation Female Fertilization in Vitro Gynecology. Andrology. Obstetrics Humans Infertility, Female - blood Intracellular Membranes - metabolism Key words: implantation failures/IVF failures/recurrent pregnancy losses/Th1/Th2 cytokines Lymphocyte Subsets - pathology Medical sciences Pregnancy Pregnancy. Fetus. Placenta T-Lymphocytes, Helper-Inducer - immunology T-Lymphocytes, Regulatory - immunology Th1 Cells - metabolism Th2 Cells - metabolism Treatment Failure implantation failures/IVF failures/recurrent pregnancy losses/Th1/Th2 cytokines Human Relapse Immune response Pregnancy disorders Cytokine Assisted procreation Female sterility implantation failures/IVF failures/recurrent pregnancy losses/Th1/lh2 cytokines Abortion Female genital diseases In vitro fertilization embryo transfer T-Lymphocyte Female
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Summary:	BACKGROUND: We aimed to study T‐helper 1 (Th1) and Th2 intracellular cytokine expression in peripheral blood lymphocytes of women with recurrent spontaneous abortions (RSA) or infertility with multiple implantation failures after IVF cycles. METHODS: Twenty‐six women with three or more RSA and 23 with two or more IVF failures (14 with no history of spontaneous abortion (SAB) and nine with more than one SAB) comprised the two study groups. Twenty‐one non‐pregnant healthy multiparous women served as controls. Proportions (%) of lymphocytes containing IFN‐γ, TNF‐α, IL‐4 and IL‐10 and the Th1/Th2 ratios of IFN‐γ/IL‐4, IFN‐γ/IL‐10, TNF‐α/IL‐4 and TNF‐α/IL‐10 in CD3+, CD3+/CD8– (T helper) and CD3+/CD8+ (T suppressor) cells were measured by 4‐colour flow cytometry. RESULTS: RSA women demonstrated significantly higher Th1/Th2 ratios of IFN‐γ/IL‐4 (P < 0.01), TNF‐α/IL‐4 and TNF‐α/IL‐10 (P < 0.05 each) in CD3+/CD8– T helper cells than those of controls. The proportion of TNF‐α producing CD3+/CD8– cells (P < 0.05), and the Th1/Th2 ratios of TNF‐α/IL‐4 (P < 0.05) and TNF‐α/IL‐10 (P < 0.005) in CD3+/CD8– cells were significantly higher in women with multiple IVF failures without SAB as compared with those of controls. CONCLUSIONS: The prevalence of dominant Th1 immune responses in peripheral blood lymphocytes may reflect the systemic contribution of Th1 cytokines to RSA or multiple implantation failures in IVF cycles.
Bibliography:	local:deg156 istex:2272F31DB5DE71545131B951F737D63C0EDCB2A5 3To whom correspondence should be addressed. e‐mail: Kwakj@finchcms.edu ark:/67375/HXZ-4SKMNDBR-K ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0268-1161 1460-2350 1460-2350
DOI:	10.1093/humrep/deg156