Filamentous fungi as cell factories for heterologous protein production

Filamentous fungi have been used as sources of metabolites and enzymes for centuries. For about two decades, molecular genetic tools have enabled us to use these organisms to express extra copies of both endogenous and exogenous genes. This review of current practice reveals that molecular tools hav...

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Bibliographic Details
Published in:	Trends in Biotechnology Vol. 20; no. 5; pp. 200 - 206
Main Authors:	Punt, Peter J, van Biezen, Nick, Conesa, Ana, Albers, Alwin, Mangnus, Jeroen, van den Hondel, Cees
Format:	Book Review Journal Article
Language:	English
Published:	Oxford Elsevier Ltd 01-05-2002 Elsevier Science Elsevier Limited
Subjects:	Aspergillus Biological and medical sciences Biotechnology Cloning controlled fermentation cytokines Enzymes Factories Fermentation - genetics Fermentation - physiology Food Fundamental and applied biological sciences. Psychology Fungi Fungi - genetics Fungi - metabolism gene technology Genes Genetic diversity Genetic engineering Genetic technics Genomes GRAS status Humans industrial enzymes Interleukin-6 - analysis Interleukin-6 - biosynthesis Interleukin-6 - genetics Metabolites Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Microorganisms Modification of gene expression level peroxidases Peroxidases - analysis Peroxidases - biosynthesis Peroxidases - genetics Protein Conformation protein secretion Proteins Recombinant Proteins - analysis Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Technological change Yeast Aspergillus Biotechnology controlled fermentation Cell biology Genetics peroxidases cytokines industrial enzymes Bioinformatics gene technology protein secretion GRAS status Human Molecular processing Enzyme Manganese peroxidase Refolding Cytokine Basidiomycetes Glycosylation Review Gene expression Fungi Interleukin 6 Peroxidases Aspergillus nidulans Phanerochaete chrysosporium Production Microorganism culture Genetic engineering Fungi Imperfecti Oxidoreductases Recombinant protein Thallophyta
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Summary:	Filamentous fungi have been used as sources of metabolites and enzymes for centuries. For about two decades, molecular genetic tools have enabled us to use these organisms to express extra copies of both endogenous and exogenous genes. This review of current practice reveals that molecular tools have enabled several new developments. But it has been process development that has driven the final breakthrough to achieving commercially relevant quantities of protein. Recent research into gene expression in filamentous fungi has explored their wealth of genetic diversity with a view to exploiting them as expression hosts and as a source of new genes. Inevitably, the progress in the ‘genomics’ technology will further develop high-throughput technologies for these organisms. Filamentous fungi have become preferred host organisms for protein production through a combination of molecular tools and process development.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 ObjectType-Review-3 content type line 23 ObjectType-Feature-3 ObjectType-Review-1
ISSN:	0167-7799 1879-3096
DOI:	10.1016/S0167-7799(02)01933-9