Search Results - "Manders, E M M"

Controlled light-exposure microscopy reduces photobleaching and phototoxicity in fluorescence live-cell imaging by Manders, E M M, Hoebe, R A, Van Oven, C H, Gadella, T W J, Dhonukshe, P B, Van Noorden, C J F

“…Fluorescence microscopy of living cells enables visualization of the dynamics and interactions of intracellular molecules. However, fluorescence live-cell…”
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A live-cell super-resolution technique demonstrated by imaging germinosomes in wild-type bacterial spores by Breedijk, R. M. P., Wen, J., Krishnaswami, V., Bernas, T., Manders, E. M. M., Setlow, P., Vischer, N. O. E., Brul, S.

“…Time-lapse fluorescence imaging of live cells at super-resolution remains a challenge, especially when the photon budget is limited. Current super-resolution…”
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Dynamics of three-dimensional replication patterns during the S-phase, analysed by double labelling of DNA and confocal microscopy by MANDERS, E. M. M, STAP, J, BRAKENHOFF, G. J, VAN DRIEL, R, ATEN, J. A

“…The temporal and spatial progression of DNA replication in interphase nuclei of eukaryotic cells has been investigated. Application of a recently developed…”
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Configurations of the Re‐scan Confocal Microscope (RCM) for biomedical applications by DE LUCA, G.M.R., DESCLOS, E., BREEDIJK, R.M.P., DOLZ‐EDO, L., SMITS, G.J., NAHIDIAZAR, L., BIELEFELD, P., PICAVET, L., FITZSIMONS, C.P., HOEBE, R., MANDERS, E.M.M.

“…Summary The new high‐sensitive and high‐resolution technique, Re‐scan Confocal Microscopy (RCM), is based on a standard confocal microscope extended with a…”
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Controlled light exposure microscopy reveals dynamic telomere microterritories throughout the cell cycle by De Vos, Winnok H, Hoebe, R.A, Joss, G.H, Haffmans, W, Baatout, S, Van Oostveldt, P, Manders, E.M.M

“…Telomeres are complex end structures that confer functional integrity and positional stability to human chromosomes. Despite their critical importance, there…”
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Combination of a spinning disc confocal unit with frequency‐domain fluorescence lifetime imaging microscopy by van Munster, E. B., Goedhart, J., Kremers, G. J., Manders, E. M. M., Gadella, T. W. J.

“…Background: Wide‐field frequency‐domain fluorescence lifetime imaging microscopy (FLIM) is an established technique to determine fluorescence lifetimes…”
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Quantitative determination of the reduction of phototoxicity and photobleaching by controlled light exposure microscopy by HOEBE, R.A, VAN DER VOORT, H.T.M, STAP, J, VAN NOORDEN, C.J.F, MANDERS, E.M.M

“…Phototoxicity and photobleaching are major limitations in live-cell fluorescence microscopy. They are caused by fluorophores in an excited singlet or triplet…”
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Noise effects and filtering in controlled light exposure microscopy by HOEBE, R.A, VAN NOORDEN, C.J.F, MANDERS, E.M.M

“…Phototoxicity and photobleaching are major limitations of fluorescence live‐cell microscopy. A straightforward way to limit phototoxicity and photobleaching is…”
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Four-dimensional telomere analysis in recordings of living human cells acquired with Controlled Light Exposure Microscopy by DE VOS, W.H, JOSS, G.H, HAFFMANS, W, HOEBE, R.A, MANDERS, E.M.M, VAN OOSTVELDT, P

“…Telomeres are the complex end structures that confer functional integrity and positional stability to human chromosomes. Telomere research has long been…”
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Evaluation of the Siemens VERSANT® CT/GC DNA 1.0 Assay (kPCR) for detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Bongaerts, Maarten, van de Bovenkamp, Jeroen H.B., Morré, Servaas A., Manders, Monique E.L.M., Heddema, Edou R.

“…The Siemens VERSANT kPCR system is an automated system which combines extraction of nucleic acids from 96 samples with subsequent real-time PCR. The VERSANT…”
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Dynamics of the nuclear lamina as monitored by GFP-tagged A-type lamins by Broers, J L, Machiels, B M, van Eys, G J, Kuijpers, H J, Manders, E M, van Driel, R, Ramaekers, F C

“…The behavior of chimeric proteins consisting of A-type lamins and green fluorescent protein (GFP) was studied to investigate the localization and dynamics of…”
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Four-dimensional imaging of chromatin dynamics during the assembly of the interphase nucleus by Manders, E M M, Visser, A E, Koppen, A, de Leeuw, W C, van Liere, R, Brakenhoff, G J, van Driel, R

“…Large-scale chromatin organization is likely to play an important role in epigenetic control of gene expression. This implies that after mitosis the correct…”
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The RNA 3′ cleavage factors CstF 64 kDa and CPSF 100 kDa are concentrated in nuclear domains closely associated with coiled bodies and newly synthesized RNA by Schul, W., Groenhout, B., Koberna, K., Takagaki, Y., Jenny, A., Manders, E. M., Raska, I., Driel, R., Jong, L.

“…The cleavage stimulation factor (CstF), and the cleavage and polyadenylation specificity factor (CPSF) are necessary for 3′‐terminal processing of…”
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Chromatic shift in multicolour confocal microscopy by Manders, E. M. M.

“…Multicolour confocal microscopy has proven to be a successful technique for the analysis of the spatial relationship between different biological structures in…”
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Spatial Relationship between Transcription Sites and Chromosome Territories by Verschure, Pernette J., van der Kraan, Ineke, Erik M. M. Manders, van Driel, Roel

“…We have investigated the spatial relationship between transcription sites and chromosome territories in the interphase nucleus of human female fibroblasts…”
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The Polycomb group protein EZH2 is upregulated in proliferating, cultured human mantle cell lymphoma by Visser, Hein P. J., Gunster, Marco J., Kluin‐Nelemans, Hanneke C., Manders, Erik M. M., Raaphorst, Frank M., Meijer, Chris J. L. M., Willemze, Roel, Otte, Arie P.

“…Polycomb group (PcG) proteins are involved in the stable transmittance of the repressive state of their gene targets throughout the cell cycle. Mis‐expression…”
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Numbers and organization of RNA polymerases, nascent transcripts, and transcription units in HeLa nuclei by Jackson, D A, Iborra, F J, Manders, E M, Cook, P R

“…Using HeLa cells, we have developed methods to determine 1) the number of RNA polymerases that are active at any moment, 2) the number of transcription sites,…”
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Direct Imaging of DNA in Living Cells Reveals the Dynamics of Chromosome Formation by Erik M. M. Manders, Kimura, Hiroshi, Cook, Peter R.

“…Individual chromosomes are not directly visible within the interphase nuclei of most somatic cells; they can only be seen during mitosis. We have developed a…”
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Local UV-induced DNA damage in cell nuclei results in local transcription inhibition by Moné, Martijn J, Volker, Marcel, Nikaido, Osamu, Mullenders, Leon H F, van Zeeland, Albert A, Verschure, Pernette J, Manders, Erik M M, van Driel, Roel

“…UV‐induced DNA damage causes cells to repress RNA synthesis and to initiate nucleotide excision repair (NER). NER and transcription are intimately linked…”
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Largest contour segmentation: A tool for the localization of spots in confocal images by Manders, E. M. M., Hoebe, R., Strackee, J., Vossepoel, A. M., Aten, J. A.

“…An accurate determination of the 3‐D positions of multiple spots in images obtained by confocal microscopy is essential for the investigation of the spatial…”
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