Search Results - "Manalis, Scott R."

Amino Acids Rather than Glucose Account for the Majority of Cell Mass in Proliferating Mammalian Cells by Hosios, Aaron M., Hecht, Vivian C., Danai, Laura V., Johnson, Marc O., Rathmell, Jeffrey C., Steinhauser, Matthew L., Manalis, Scott R., Vander Heiden, Matthew G.

“…Cells must duplicate their mass in order to proliferate. Glucose and glutamine are the major nutrients consumed by proliferating mammalian cells, but the…”
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A comparison of microfluidic methods for high-throughput cell deformability measurements by Urbanska, Marta, Muñoz, Hector E., Shaw Bagnall, Josephine, Otto, Oliver, Manalis, Scott R., Di Carlo, Dino, Guck, Jochen

“…The mechanical phenotype of a cell is an inherent biophysical marker of its state and function, with many applications in basic and applied biological…”
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High-speed multiple-mode mass-sensing resolves dynamic nanoscale mass distributions by Olcum, Selim, Cermak, Nathan, Wasserman, Steven C., Manalis, Scott R.

“…Simultaneously measuring multiple eigenmode frequencies of nanomechanical resonators can determine the position and mass of surface-adsorbed proteins, and…”
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Characterizing deformability and surface friction of cancer cells by Byun, Sangwon, Son, Sungmin, Amodei, Dario, Cermak, Nathan, Shaw, Josephine, Kang, Joon Ho, Hecht, Vivian C., Winslow, Monte M., Jacks, Tyler, Mallick, Parag, Manalis, Scott R.

“…Metastasis requires the penetration of cancer cells through tight spaces, which is mediated by the physical properties of the cells as well as their…”
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High-throughput measurement of single-cell growth rates using serial microfluidic mass sensor arrays by Cermak, Nathan, Olcum, Selim, Delgado, Francisco Feijó, Wasserman, Steven C, Payer, Kristofor R, A Murakami, Mark, Knudsen, Scott M, Kimmerling, Robert J, Stevens, Mark M, Kikuchi, Yuki, Sandikci, Arzu, Ogawa, Masaaki, Agache, Vincent, Baléras, François, Weinstock, David M, Manalis, Scott R

“…Heterogeneity in growth phenotypes and drug susceptibility in bacterial and mammalian cells are assayed at the single-cell level using multiplexed resonant…”
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Pyruvate Kinase Isoform Expression Alters Nucleotide Synthesis to Impact Cell Proliferation by Lunt, Sophia Y., Muralidhar, Vinayak, Hosios, Aaron M., Israelsen, William J., Gui, Dan Y., Newhouse, Lauren, Ogrodzinski, Martin, Hecht, Vivian, Xu, Kali, Acevedo, Paula N. Marín, Hollern, Daniel P., Bellinger, Gary, Dayton, Talya L., Christen, Stefan, Elia, Ilaria, Dinh, Anh T., Stephanopoulos, Gregory, Manalis, Scott R., Yaffe, Michael B., Andrechek, Eran R., Fendt, Sarah-Maria, Vander Heiden, Matthew G.

“…Metabolic regulation influences cell proliferation. The influence of pyruvate kinase isoforms on tumor cells has been extensively studied, but whether PKM2 is…”
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Direct observation of mammalian cell growth and size regulation by Son, Sungmin, Tzur, Amit, Weng, Yaochung, Jorgensen, Paul, Kim, Jisoo, Kirschner, Marc W, Manalis, Scott R

“…Precise mass measurement on single mammalian cells is combined with imaging to monitor changes in cellular mass during the cell cycle over many generations. We…”
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Noninvasive monitoring of single-cell mechanics by acoustic scattering by Kang, Joon Ho, Miettinen, Teemu P., Chen, Lynna, Olcum, Selim, Katsikis, Georgios, Doyle, Patrick S., Manalis, Scott R.

“…The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify…”
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Mass measurements during lymphocytic leukemia cell polyploidization decouple cell cycle- and cell size-dependent growth by Mu, Luye, Kang, Joon Ho, Olcum, Selim, Payer, Kristofor R., Calistri, Nicholas L., Kimmerling, Robert J., Manalis, Scott R., Miettinen, Teemu P.

“…Cell size is believed to influence cell growth and metabolism. Consistently, several studies have revealed that large cells have lower mass accumulation rates…”
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Measuring single-cell density by Grover, William H, Bryan, Andrea K, Diez-Silva, Monica, Suresh, Subra, Higgins, John M, Manalis, Scott R

“…We have used a microfluidic mass sensor to measure the density of single living cells. By weighing each cell in two fluids of different densities, our…”
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Weighing of biomolecules, single cells and single nanoparticles in fluid by Carlson, Greg, Burg, Thomas P, Godin, Michel, Foster, John S, Knudsen, Scott M, Shen, Wenjiang, Manalis, Scott R, Babcock, Ken

“…Nanomechanical resonators enable the measurement of mass with extraordinary sensitivity. Previously, samples as light as 7 zeptograms (1 zg  =  10-21 g) have…”
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Intracellular water exchange for measuring the dry mass, water mass and changes in chemical composition of living cells by Feijó Delgado, Francisco, Cermak, Nathan, Hecht, Vivian C, Son, Sungmin, Li, Yingzhong, Knudsen, Scott M, Olcum, Selim, Higgins, John M, Chen, Jianzhu, Grover, William H, Manalis, Scott R

“…We present a method for direct non-optical quantification of dry mass, dry density and water mass of single living cells in suspension. Dry mass and dry…”
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Measurement of mass, density, and volume during the cell cycle of yeast by Bryan, Andrea K, Goranov, Alexi, Amon, Angelika, Manalis, Scott R

“…Cell growth comprises changes in both mass and volume--two processes that are distinct, yet coordinated through the cell cycle. Understanding this relationship…”
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A microfluidic platform enabling single-cell RNA-seq of multigenerational lineages by Kimmerling, Robert J., Lee Szeto, Gregory, Li, Jennifer W., Genshaft, Alex S., Kazer, Samuel W., Payer, Kristofor R., de Riba Borrajo, Jacob, Blainey, Paul C., Irvine, Darrell J., Shalek, Alex K., Manalis, Scott R.

“…We introduce a microfluidic platform that enables off-chip single-cell RNA-seq after multi-generational lineage tracking under controlled culture conditions…”
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Using buoyant mass to measure the growth of single cells by Kirschner, Marc W, Son, Sungmin, Grover, William H, Bryan, Andrea K, Jorgensen, Paul, Manalis, Scott R, Delgado, Francisco Feijó, Godin, Michel, Payer, Kris, Grossman, Alan D, Tzur, Amit

“…We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous'…”
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Deformability of Tumor Cells versus Blood Cells by Shaw Bagnall, Josephine, Byun, Sangwon, Begum, Shahinoor, Miyamoto, David T., Hecht, Vivian C., Maheswaran, Shyamala, Stott, Shannon L., Toner, Mehmet, Hynes, Richard O., Manalis, Scott R.

“…The potential for circulating tumor cells (CTCs) to elucidate the process of cancer metastasis and inform clinical decision-making has made their isolation of…”
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Characterizing Cellular Biophysical Responses to Stress by Relating Density, Deformability, and Size by Byun, Sangwon, Hecht, Vivian C., Manalis, Scott R.

“…Cellular physical properties are important indicators of specific cell states. Although changes in individual biophysical parameters, such as cell size,…”
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Alveolar proteins stabilize cortical microtubules in Toxoplasma gondii by Harding, Clare R., Gow, Matthew, Kang, Joon Ho, Shortt, Emily, Manalis, Scott R., Meissner, Markus, Lourido, Sebastian

“…Single-celled protists use elaborate cytoskeletal structures, including arrays of microtubules at the cell periphery, to maintain polarity and rigidity. The…”
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Single-cell monitoring of dry mass and dry mass density reveals exocytosis of cellular dry contents in mitosis by Miettinen, Teemu P, Ly, Kevin S, Lam, Alice, Manalis, Scott R

“…Cell mass and composition change with cell cycle progression. Our previous work characterized buoyant mass dynamics in mitosis (Miettinen et al., 2019), but…”
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Variations of intracellular density during the cell cycle arise from tip-growth regulation in fission yeast by Odermatt, Pascal D, Miettinen, Teemu P, Lemière, Joël, Kang, Joon Ho, Bostan, Emrah, Manalis, Scott R, Huang, Kerwyn Casey, Chang, Fred

“…Intracellular density impacts the physical nature of the cytoplasm and can globally affect cellular processes, yet density regulation remains poorly…”
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