Comparative electron microscopic study on projectin and titin binding to F-actin

Comparative electron microscopic study on projectin and titin binding to F-actin

Using the system of F-actin paracrystals, we have obtained electron microscopic evidence that projectin from synchronous flight muscles of Locusta migratoria binds to actin filaments in the same fashion as skeletal titin. Control actin paracrystals formed in the presence of Mg 2+ ions have great wid...

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Bibliographic Details
Published in:Insect biochemistry and molecular biology Vol. 33; no. 8; pp. 789 - 793
Main Authors: Podlubnaya, Z.A, Shpagina, M.D, Vikhlyantsev, I.M, Malyshev, S.L, Udaltsov, S.N, Ziegler, C, Beinbrech, G
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01-08-2003
Subjects:
Actins - metabolism
Animals
Connectin
Electron microscopy
F-actin paracrystals
Flight, Animal
Grasshoppers - physiology
Locusta migratoria
Microscopy, Electron
Muscle Proteins - chemistry
Muscle Proteins - metabolism
Muscles - physiology
Projectin
Protein Kinases - chemistry
Protein Kinases - metabolism
Titin
Projectin
Locusta migratoria
Electron microscopy
Titin
F-actin paracrystals
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Summary:Using the system of F-actin paracrystals, we have obtained electron microscopic evidence that projectin from synchronous flight muscles of Locusta migratoria binds to actin filaments in the same fashion as skeletal titin. Control actin paracrystals formed in the presence of Mg 2+ ions have great width and length and blunted ends. The addition of either projectin or titin results in disruption of compact ordered packing of F-actin in paracrystals and leads to the formation of loose filament bundles with smaller diameters and tapered ends. It is also accompanied with the appearance of individual actin filaments in considerable amounts. The effect becomes more pronounced with the increase in concentrations of added projectin or titin. Possible physiological implications of projectin–actin interactions are discussed.
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ISSN:0965-1748
1879-0240
DOI:10.1016/S0965-1748(03)00077-8