Sialyltransferase activity of human plasma and aortic intima is enhanced in atherosclerosis

Sialyltransferase activity of human plasma and aortic intima is enhanced in atherosclerosis

Sialyltransferase activity has been determined in membrane preparations containing the Golgi apparatus that were isolated from atherosclerotic and normal human aortic intima as well as in plasma of patients with documented atherosclerosis and healthy donors by measuring the transfer of N-acetylneura...

Full description

Saved in:
Bibliographic Details
Published in:Biochimica et biophysica acta Vol. 1586; no. 1; pp. 123 - 128
Main Authors: Gracheva, Elena V, Samovilova, Nelya N, Golovanova, Natalia K, Il’inskaya, Olga P, Tararak, Edward M, Malyshev, Peter P, Kukharchuk, Valery V, Prokazova, Nina V
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 02-01-2002
Subjects:
Adult
Aged
alpha-Fetoproteins - metabolism
Aorta - enzymology
Aorta - pathology
Arteriosclerosis - blood
Arteriosclerosis - enzymology
Arteriosclerosis - pathology
Asialoglycoproteins - metabolism
Atherosclerosis
Coronary Vessels - enzymology
Coronary Vessels - pathology
Female
Fetuins
Human aortic intima
Human blood plasma
Humans
Kinetics
Male
Membrane fraction
Middle Aged
N-Acetylneuraminic Acid - metabolism
Sialyltransferase activity
Sialyltransferases - metabolism
Tunica Intima - enzymology
Up-Regulation
Sialyltransferase activity
Human blood plasma
Human aortic intima
Membrane fraction
Atherosclerosis
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Sialyltransferase activity has been determined in membrane preparations containing the Golgi apparatus that were isolated from atherosclerotic and normal human aortic intima as well as in plasma of patients with documented atherosclerosis and healthy donors by measuring the transfer of N-acetylneuraminic acid (NeuAc) from CMP-NeuAc to asialofetuin. The asialofetuin sialyltransferase activity was found to be 2 times higher in the atherosclerotic intima as compared to the normal intima and 2-fold higher in patients’ plasma than in that from healthy donors. The mean values of the apparent Michaelis constant ( K m) for the sialylating enzyme for both tissues did not differ and were close for the intima and plasma. In contrast, the maximal velocity ( V max) was 2 times higher for the atherosclerotic intima than for the normal intima and 3 times higher for patients’ plasma than for that of the donors. These results suggest that the activity of asialofetuin sialyltransferases of aortal intima is enhanced in atherosclerosis as is the secretion of their soluble forms into patients’ plasma.
ISSN:0925-4439
0006-3002
1879-260X
DOI:10.1016/S0925-4439(01)00093-X