Heparanase 1: a key participant of inner root sheath differentiation program and hair follicle homeostasis

: Heparanase is a heparan sulphate endo‐glycosidase which was previously detected in the outer root sheath of murine hair follicles. Heparanase overexpression was reported to improve mouse hair (re)growth. In this study, we investigated its involvement in human hair biology. Immunofluorescence dete...

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Bibliographic Details
Published in:	Experimental dermatology Vol. 17; no. 12; pp. 1017 - 1023
Main Authors:	Malgouries, Sylvain, Donovan, Mark, Thibaut, Sébastien, Bernard, Bruno A.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 01-12-2008 Blackwell
Subjects:	Aged Biological and medical sciences Cell Differentiation - physiology Dermatology Desmoglein 1 - metabolism Enzyme Inhibitors - pharmacology Epidermis - metabolism Epithelial Cells - cytology Epithelial Cells - drug effects Epithelial Cells - metabolism Female Fibronectins - metabolism hair Hair Follicle - cytology Hair Follicle - drug effects Hair Follicle - physiology heparan sulfate heparanase 1 Heparin Lyase - antagonists & inhibitors Heparin Lyase - metabolism Heparitin Sulfate - metabolism Homeostasis - physiology Humans Immunohistochemistry inner root sheath Keratin-14 - metabolism Keratins, Hair-Specific - metabolism Keratins, Type I - metabolism Ki-67 Antigen - metabolism Medical sciences Middle Aged Tissue Culture Techniques Transglutaminases - metabolism Hair Program Dermatology Homeostasis Hair follicle heparanase 1 Cell differentiation Sheath Hair (head) heparan sulfate Heparanase Heparitin sulfate inner root sheath Differentiation
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Summary:	: Heparanase is a heparan sulphate endo‐glycosidase which was previously detected in the outer root sheath of murine hair follicles. Heparanase overexpression was reported to improve mouse hair (re)growth. In this study, we investigated its involvement in human hair biology. Immunofluorescence detection was used to explore heparanase distribution in both anagen and catagen hair follicles. Heparanase functionality was assessed in in vitro cultured hair follicles, in the presence of a heparanase activity inhibitor. Our results showed that heparanase expression was (i) primarily located in the inner root sheath (IRS) of human hair follicle, and there (ii) restricted to anagen phase. Furthermore, inhibition of heparanase in in vitro cultured hair follicles induced a catagen‐like process. Hair shaft retreat upward was accompanied by a decrease in Ki67‐positive cells, the formation of an epithelial strand as evidenced by K14 keratin expression, and the loss of IRS as assessed by transglutaminase 1 and desmoglein labelling. IRS distribution of heparanase and the induction of catagen‐like involution of hair follicles when a potent heparanase inhibitor is added suggest that heparanase is a key actor of IRS differentiation and hair homeostasis.
Bibliography:	istex:C6F4D7CB016C41AC0960224C559FD3AC9B53D630 ark:/67375/WNG-V3DQGVS0-Q ArticleID:EXD739 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0906-6705 1600-0625
DOI:	10.1111/j.1600-0625.2008.00739.x