Search Results - "Malacrida, Leonel"

Fit-free analysis of fluorescence lifetime imaging data using the phasor approach by Ranjit, Suman, Malacrida, Leonel, Jameson, David M., Gratton, Enrico

“…Fluorescence lifetime imaging microscopy (FLIM) is used in diverse disciplines, including biology, chemistry and biophysics, but its use has been limited by…”
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Linear Combination Properties of the Phasor Space in Fluorescence Imaging by Torrado, Belén, Malacrida, Leonel, Ranjit, Suman

“…The phasor approach to fluorescence lifetime imaging, and more recently hyperspectral fluorescence imaging, has increased the use of these techniques, and…”
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Multi-harmonic spectral phasor analysis of LAURDAN hyperspectral imaging enables the in vivo study of membrane dynamics of multiple membrane-bound organelles by Vallmitjana, Alexander, Lepanto, Paola, Irigoin, Florencia, Malacrida, Leonel S.

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A global view of standards for open image data formats and repositories by Swedlow, Jason R., Kankaanpää, Pasi, Sarkans, Ugis, Goscinski, Wojtek, Galloway, Graham, Malacrida, Leonel, Sullivan, Ryan P., Härtel, Steffen, Brown, Claire M., Wood, Christopher, Keppler, Antje, Paina, Federica, Loos, Ben, Zullino, Sara, Longo, Dario Livio, Aime, Silvio, Onami, Shuichi

“…Imaging technologies are used throughout the life and biomedical sciences to understand mechanisms in biology and diagnosis and therapy in animal and human…”
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Determination of the metabolic index using the fluorescence lifetime of free and bound nicotinamide adenine dinucleotide using the phasor approach by Ranjit, Suman, Malacrida, Leonel, Stakic, Milka, Gratton, Enrico

“…The fluorescence lifetime of nicotinamide adenine dinucleotide (NADH) is commonly used in conjunction with the phasor approach as a molecular biomarker to…”
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Biomolecular condensates modulate membrane lipid packing and hydration by Mangiarotti, Agustín, Siri, Macarena, Tam, Nicky W., Zhao, Ziliang, Malacrida, Leonel, Dimova, Rumiana

“…Membrane wetting by biomolecular condensates recently emerged as a key phenomenon in cell biology, playing an important role in a diverse range of processes…”
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A multidimensional phasor approach reveals LAURDAN photophysics in NIH-3T3 cell membranes by Malacrida, Leonel, Jameson, David M., Gratton, Enrico

“…Mammalian cell membranes have different phospholipid composition and cholesterol content, displaying a profile of fluidity that depends on their intracellular…”
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PTEN Deficiency and AMPK Activation Promote Nutrient Scavenging and Anabolism in Prostate Cancer Cells by Kim, Seong M, Nguyen, Tricia T, Ravi, Archna, Kubiniok, Peter, Finicle, Brendan T, Jayashankar, Vaishali, Malacrida, Leonel, Hou, Jue, Robertson, Jane, Gao, Dong, Chernoff, Jonathan, Digman, Michelle A, Potma, Eric O, Tromberg, Bruce J, Thibault, Pierre, Edinger, Aimee L

“…We report that PTEN-deficient prostate cancer cells use macropinocytosis to survive and proliferate under nutrient stress. PTEN loss increased macropinocytosis…”
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Phasor-based hyperspectral snapshot microscopy allows fast imaging of live, three-dimensional tissues for biomedical applications by Hedde, Per Niklas, Cinco, Rachel, Malacrida, Leonel, Kamaid, Andrés, Gratton, Enrico

“…Hyperspectral imaging is highly sought after in many fields including mineralogy and geology, environment and agriculture, astronomy and, importantly,…”
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Measurements of absolute concentrations of NADH in cells using the phasor FLIM method by Ma, Ning, Digman, Michelle A, Malacrida, Leonel, Gratton, Enrico

“…We propose a graphical method using the phasor representation of the fluorescence decay to derive the absolute concentration of NADH in cells. The method…”
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Spectral phasor analysis of LAURDAN fluorescence in live A549 lung cells to study the hydration and time evolution of intracellular lamellar body-like structures by Malacrida, Leonel, Astrada, Soledad, Briva, Arturo, Bollati-Fogolín, Mariela, Gratton, Enrico, Bagatolli, Luis A.

“…Using LAURDAN spectral imaging and spectral phasor analysis we concurrently studied the growth and hydration state of subcellular organelles (lamellar…”
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Label-free fluorescence microscopy: revisiting the opportunities with autofluorescent molecules and harmonic generations as biosensors and biomarkers for quantitative biology by García, María José, Kamaid, Andrés, Malacrida, Leonel

“…Over the past decade, the utilization of advanced fluorescence microscopy technologies has presented numerous opportunities to study or re-investigate…”
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Novel opportunities from bioimaging to understand the trafficking and maturation of intracellular pulmonary surfactant and its role in lung diseases by Garcia, María José, Amarelle, Luciano, Malacrida, Leonel, Briva, Arturo

“…Pulmonary surfactant (PS), a complex mixture of lipids and proteins, is essential for maintaining proper lung function. It reduces surface tension in the…”
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Hyperoxia and Lungs: What We Have Learned From Animal Models by Amarelle, Luciano, Quintela, Lucía, Hurtado, Javier, Malacrida, Leonel

“…Although oxygen (O ) is essential for aerobic life, it can also be an important source of cellular damage. Supra-physiological levels of O determine toxicity…”
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StarD5: an ER stress protein regulates plasma membrane and intracellular cholesterol homeostasis by Rodriguez-Agudo, Daniel, Malacrida, Leonel, Kakiyama, Genta, Sparrer, Tavis, Fortes, Carolina, Maceyka, Michael, Subler, Mark A., Windle, Jolene J., Gratton, Enrico, Pandak, William M., Gil, Gregorio

“…How plasma membrane (PM) cholesterol is controlled is poorly understood. Ablation of the gene encoding the ER stress steroidogenic acute regulatory-related…”
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The DIVER Microscope for Imaging in Scattering Media by Dvornikov, Alexander, Malacrida, Leonel, Gratton, Enrico

“…We describe an advanced DIVER (Deep Imaging Via Emission Recovery) detection system for two-photon fluorescence microscopy that allows imaging in multiple…”
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Quantitative melanoma diagnosis using spectral phasor analysis of hyperspectral imaging from label-free slices by Schuty, Bruno, Martínez, Sofía, Guerra, Analía, Lecumberry, Federico, Magliano, Julio, Malacrida, Leonel

“…Melanoma diagnosis traditionally relies on microscopic examination of hematoxylin and eosin (H&E) slides by dermatopathologists to search for specific…”
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Fluorescence Lifetime Phasor Analysis of the Decamer-Dimer Equilibrium of Human Peroxiredoxin 1 by Villar, Sebastián F, Dalla-Rizza, Joaquín, Möller, Matías N, Ferrer-Sueta, Gerardo, Malacrida, Leonel, Jameson, David M, Denicola, Ana

“…Protein self-assembly is a common feature in biology and is often required for a myriad of fundamental processes, such as enzyme activity, signal transduction,…”
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Publisher Correction: Alteration in Fluidity of Cell Plasma Membrane in Huntington Disease Revealed by Spectral Phasor Analysis by Sameni, Sara, Malacrida, Leonel, Tan, Zhiqun, Digman, Michelle A.

“…A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper…”
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Phasor plots and the future of spectral and lifetime imaging by Malacrida, Leonel

“…I share my opinions on the benefits of and bottlenecks for hyperspectral and time-resolved imaging. I also discuss current and future perspectives for…”
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