Disease status in patients with chronic myeloid leukemia is better characterized by BCR-ABL/BCR transcript ratio than by BCR-ABL transcript level, which may suggest a role of normal BCR gene in the disease pathogenesis

Monitoring of BCR-ABL transcript level is widely used in chronic myeloid leukemia (CML) to follow up response to therapy. In this study we compare abilities of two quantitative RT-PCR assays to characterize the disease status in CML patients: RT-PCR quantifying the BCR-ABL transcript concentration a...

Full description

Saved in:

Bibliographic Details
Published in:	Neoplasma Vol. 52; no. 2; p. 119
Main Authors:	Moravcova, J, Regner, J, Mouckova, D, Fiser, K, Zmekova, V, Malacova, R, Michalova, K, Klamova, H
Format:	Journal Article
Language:	English
Published:	Slovakia 2005
Subjects:	Adult Aged Cell Transformation, Neoplastic Disease Progression Female Genes, abl Humans Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics Leukemia, Myelogenous, Chronic, BCR-ABL Positive - pathology Longitudinal Studies Male Middle Aged Prognosis Protein-Tyrosine Kinases - biosynthesis Proto-Oncogene Proteins - biosynthesis Proto-Oncogene Proteins c-bcr Reverse Transcriptase Polymerase Chain Reaction
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Monitoring of BCR-ABL transcript level is widely used in chronic myeloid leukemia (CML) to follow up response to therapy. In this study we compare abilities of two quantitative RT-PCR assays to characterize the disease status in CML patients: RT-PCR quantifying the BCR-ABL transcript concentration and RT-PCR determining the BCR-ABL/BCR transcript ratio (R). We demonstrate that in non-responders only R, but not BCR-ABL, unambiguously characterizes the state of disease. Moreover, R values >1 found in all poor responders indicate lower BCR expression compared to BCR- ABL in these patients. Our results demonstrate the importance of BCR-ABL/BCR transcript ratio for the disease status and the disease prognosis characterization and suggest a possible role of the normal BCR gene expression in CML pathogenesis.
ISSN:	0028-2685