Effects of estradiol on in vitro maturation of buffalo and goat oocytes

Effects of estradiol on in vitro maturation of buffalo and goat oocytes

Purpose The effects of estradiol on oocyte development seem to be varied among species. The present study investigated the effects of 17β‐estradiol on in vitro maturation of buffalo and goat oocytes. Methods Cumulus oocyte complexes (COCs) were aspirated from large antral follicles of slaughtered bu...

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Published in:Reproductive medicine and biology Vol. 20; no. 1; pp. 62 - 70
Main Authors: Maksura, Hurum, Akon, Narsisa, Islam, Md Nuronnabi, Akter, Ireen, Modak, Avijit Kumar, Khatun, Asma, Alam, Md Hasanur, Hashem, Md Abul, Amin, Md Ruhul, Moniruzzaman, Mohammad
Format: Journal Article
Language:English
Published: Japan John Wiley & Sons, Inc 01-01-2021
John Wiley and Sons Inc
Subjects:
17β-Estradiol
Analysis
buffalo
Cattle
Estradiol
Ethanol
Follicles
goat
Goats
in vitro maturation
Maturation
Meiosis
Metaphase
Morphology
oocyte
Oocytes
Original
Ovaries
Ovulation
Phenols
Bangladesh
in vitro maturation
oocyte
goat
17β‐estradiol
buffalo
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Summary:Purpose The effects of estradiol on oocyte development seem to be varied among species. The present study investigated the effects of 17β‐estradiol on in vitro maturation of buffalo and goat oocytes. Methods Cumulus oocyte complexes (COCs) were aspirated from large antral follicles of slaughtered buffalo and goat ovaries. COCs were cultured in TCM‐199 medium supplemented with 0, 0.5, 1, and 1.5 µg/mL of 17β‐estradiol for in vitro maturation. Then, oocytes were used for the examination of state of nuclear maturation and cumulus expansion. Results In both species, oocytes treated with 17β‐estradiol showed higher cumulus expansion rate than control (0 µg/mL treated). In buffalo, the percentage of oocytes matured to the metaphase II (MII) stage increased in the concentration‐dependent manner of 17β‐estradiol. Similarly, estradiol positively influenced nuclear maturation of goat oocytes in vitro. Conclusions Estradiol has promoting effects on normalprogress of in vitro oocyte meiosis in buffalos and goats. The effects of estradiol on oocyte maturation, ovulation, and embryo development seem to be species‐dependent. The present study investigated the effects of 17β‐estradiol on in vitro maturation in buffalo and goat oocytes. The results showed that estradiol enhanced meiotic progression of both buffalo and goat oocytes in vitro.
Bibliography:Funding information
This work was supported by Bangladesh Academy of Science (BAS), Ministry of Science and Technology of Bangladesh and United States Department of Agriculture (USDA; Grant No.: LS‐16/2017) to M. M.
Hurum Maksura, Narsisa Akon, Md Nuronnabi Islam contributed equally to this work.
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ISSN:1445-5781
1447-0578
DOI:10.1002/rmb2.12350