Search Results - "Makris, Melissa"

Optimized protocol for mouse footpad immune cell isolation for single-cell RNA sequencing and flow cytometry by Hameed, Muddassar, Rai, Pallavi, Makris, Melissa, Weger-Lucarelli, James

“…Single-cell RNA sequencing (scRNA-seq) requires the preparation of a highly viable single-cell suspension to get reliable sequencing results. Here, we present…”
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Production of interspecies somatic/pluripotent heterokaryons using polyethylene glycol (PEG) and selection by imaging flow cytometry for the study of nuclear reprogramming by Villafranca, Cristina, Makris, Melissa R., Garrido Bauerle, Maria Jesus, Jensen, Roderick V., Eyestone, Willard H.

“…Fusion of somatic cells to embryonic stem cells induces reprogramming of the somatic nucleus and can be used to study the effect of trans-acting factors from…”
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Heat Stress Increases Mammary Epithelial Cells and Reduces Viable Immune Cells in Milk of Dairy Cows by Lengi, Andrea J, Stewart, Jacob W, Makris, Melissa, Rhoads, Michelle L, Corl, Benjamin A

“…Somatic cells normally found in milk are generally either immune cells such as lymphocytes, monocytes and granulocytes, or mammary epithelial cells. The number…”
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Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow by Liao, Xiaofeng, Makris, Melissa, Luo, Xin M.

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Role of TLRs in Brucella mediated murine DC activation in vitro and clearance of pulmonary infection in vivo by Surendran, Naveen, Hiltbold, Elizabeth M, Heid, Bettina, Akira, Shizuo, Standiford, Theodore J, Sriranganathan, Nammalwar, Boyle, Stephen M, Zimmerman, Kurt L, Makris, Melissa R, Witonsky, Sharon G

“…Highlights ► The role of TLRs in Brucella mediated respiratory challenge is unknown. ► B. abortus strain RB51 stimulates TLR2, TLR4 and TLR9 dependent DC…”
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Protoplast isolation prior to flow cytometry reveals clear patterns of endoreduplication in potato tubers, related species, and some starchy root crops by Laimbeer, F Parker E, Holt, Sarah H, Makris, Melissa, Hardigan, Michael Alan, Robin Buell, C, Veilleux, Richard E

“…Endoreduplication, the process of DNA replication in the absence of cell division, is associated with specialized cellular function and increased cell size…”
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Live Brucella abortus rough vaccine strain RB51 stimulates enhanced innate immune response in vitro compared to rough vaccine strain RB51SOD and virulent smooth strain 2308 in murine bone marrow-derived dendritic cells by Surendran, Naveen, Hiltbold, Elizabeth M., Heid, Bettina, Sriranganathan, Nammalwar, Boyle, Stephen M., Zimmerman, Kurt L., Makris, Melissa R., Witonsky, Sharon. G.

“…Brucella spp. are Gram-negative, coccobacillary, facultative intracellular pathogens. B. abortus strain 2308 is a pathogenic strain affecting cattle and…”
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CD38 induces apoptosis of a murine pro-B leukemic cell line by a tyrosine kinase-dependent but ADP-ribosyl cyclase- and NAD glycohydrolase-independent mechanism by Lund, Frances E., Muller-Steffner, Hélène, Romero-Ramirez, Héctor, Moreno-García, Miguel E., Partida-Sánchez, Santiago, Makris, Melissa, Oppenheimer, Norman J., Santos-Argumedo, Leopoldo, Schuber, Francis

“…Cross-linking of CD38 on hematopoietic cells induces activation, proliferation and differentiation of mature T and B cells and mediates apoptosis of myeloid…”
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Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow by Liao, Xiaofeng, Makris, Melissa, Luo, Xin M

“…Fluorescence-activated cell sorting (FACS) is a technique to purify specific cell populations based on phenotypes detected by flow cytometry. This method…”
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Cytokine-Producing Effector B Cells Regulate Type 2 Immunity to H. polygyrus by Wojciechowski, Wojciech, Harris, David P., Sprague, Frank, Mousseau, Betty, Makris, Melissa, Kusser, Kim, Honjo, Tasuko, Mohrs, Katja, Mohrs, Markus, Randall, Troy, Lund, Frances E.

“…Immunity to the intestinal parasite Heligomosomoides polygyrus is dependent on the successful generation of T helper 2 (Th2) memory cells. We showed that B…”
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Microbial diversity of individual raindrops collected from simulated and natural precipitation events by Garcia, Ellen B., Hanlon, Regina, Makris, Melissa R., Powers, Craig W., Jimenez-Sanchez, Celia, Karatum, Osman, Marr, Linsey C., Sands, David C., Schmale, David G.

“…Precipitation samples collected at or near the surface of the earth are composite samples of many different raindrops. Little is known about the abiotic and…”
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Measuring Endoreduplication by Flow Cytometry of Isolated Tuber Protoplasts by Laimbeer, F. Parker E., Makris, Melissa, Veilleux, Richard E.

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Measuring Endoreduplication by Flow Cytometry of Isolated Tuber Protoplasts by Laimbeer, F Parker E, Makris, Melissa, Veilleux, Richard E

“…Endoreduplication, the replication of a cell's nuclear genome without subsequent cytokinesis, yields cells with increased DNA content and is associated with…”
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CD4 T Cell-Independent Antibody Response Promotes Resolution of Primary Influenza Infection and Helps to Prevent Reinfection by Lee, Byung O, Rangel-Moreno, Javier, Moyron-Quiroz, Juan E, Hartson, Louise, Makris, Melissa, Sprague, Frank, Lund, Frances E, Randall, Troy D

“…It is generally believed that the production of influenza-specific IgG in response to viral infection is dependent on CD4 T cells. However, we previously…”
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The significance of TLRs in Brucella mediated dendritic cell activation in vitro and pulmonary clearance in vivo (42.16) by Surendran, Naveen, Hiltbold, Elizabeth, Sriranganathan, Nammalwar, Heid, Bettina, Boyle, Stephen, Makris, Melissa, Zimmerman, Kurt, Witonsky, Sharon

“…Brucellosis is a worldwide zoonoses affecting 500,000 people annually with no approved human vaccines available. Live attenuated B. abortus vaccine strain RB51…”
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Role of TLRs inBrucellamediated murine DC activationin vitroand clearance of pulmonary infectionin vivo by Surendran, Naveen, Hiltbold, Elizabeth M, Heid, Bettina, Akira, Shizuo, Standiford, Theodore J, Sriranganathan, Nammalwar, Boyle, Stephen M, Zimmerman, Kurt L, Makris, Melissa R, Witonsky, Sharon G

“…Highlights * The role of TLRs inBrucellamediated respiratory challenge is unknown. *B. abortusstrain RB51 stimulates TLR2, TLR4 and TLR9 dependent DC…”
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Leukocyte adhesion deficiency type I in a mixed-breed dog by Zimmerman, Kurt L, McMillan, Kate, Monroe, WIlliam E, Sponenberg, D. Phillip, Evans, Nick, Makris, Melissa, Hammond, Sarah H, Mullarky, Isis Kanevsky, Boudreaux, Mary K

“…A 6-month-old, neutered male, mixed-breed dog was examined for a 2-month persistent fever, nonhealing dermal metacarpal area wound, and leukocytosis…”
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Regulation of type 2 immunity to H. polygyrus by effector B cells: Requirement for cytokine-producing B cells by Wojciechowski, Wojciech, Harris, David P., Sprague, Frank, Mousseau, Betty, Makris, Melissa, Kusser, Kim, Honjo, Tasuko, Mohrs, Katja, Mohrs, Markus, Randall, Troy, Lund, Frances E.

“…Immunity to the intestinal parasite, Heligomosomoides polygyrus , is dependent on the successful generation of Th2 memory cells. We show that B cells…”
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Lipid Accumulation and Dendritic Cell Function in Cancer (49.10) by Herber, Donna L., Kroeger, Jodi L., Makris, Melissa, Gabrilovich, Dmitry I.

“…Immune cell dysfunction in cancer is an area of intense study, with particular focus on dendritic cell (DC) abnormalities. The mechanism of these abnormalities…”
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A Monte Carlo Approach to Change Point Detection in a Liver Transplant Population by Athienitis-Makris, Alexia Melissa

“…Patient survival post liver transplant (LT) is important to both the patient and the center's accreditation, but over the years physicians have noticed that…”
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