Search Results - "Makrides, S. C"

Strategies for achieving high-level expression of genes in Escherichia coli by MAKRIDES, S. C

“…Progress in our understanding of several biological processes promises to broaden the usefulness of Escherichia coli as a tool for gene expression. There is an…”
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Strategies for optimizing heterologous protein expression in Escherichia coli by Hannig, Gerhard, Makrides, Savvas C

“…The many advantages of Escherichia coli have ensured that it remains a valuable host for the efficient, cost-effective and high-level production of…”
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Therapeutic inhibition of the complement system by Makrides, S C

“…The use of powerful methodologies in molecular biology, biochemistry, and physiology in the last 2 decades had led to impressive progress in our understanding…”
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Antiinflammatory Effects of Soluble Complement Receptor Type 1 Promote Rapid Recovery of Ischemia/Reperfusion Injury in Rat Small Intestine by Eror, Alec T., Stojadinovic, Alexander, Starnes, Benjamin W., Makrides, Savvas C., Tsokos, George C., Shea-Donohue, Terez

“…We examined the effect of soluble complement receptor type 1 (sCR1) on mucosal injury and inflammation in a rat model of ischemia/reperfusion. Groups of…”
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Extended in vivo half-life of human soluble complement receptor type 1 fused to a serum albumin-binding receptor by Makrides, S C, Nygren, P A, Andrews, B, Ford, P J, Evans, K S, Hayman, E G, Adari, H, Uhlén, M, Toth, C A

“…A new approach has been used to extend the T(1/2) of human soluble complement receptor type 1 (sCR1) in rats. The albumin-binding domains B2A3 (BA) and…”
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Cell surface expression of the C3b/C4b receptor (CR1) protects Chinese hamster ovary cells from lysis by human complement by S C Makrides, S M Scesney, P J Ford, K S Evans, G R Carson, H C Marsh, Jr

“…The C3b/C4b receptor, also known as complement receptor type 1 (CR1, CD35), is a single chain glycoprotein consisting of 30 repeating homologous protein…”
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Quantitative analysis of C4b dimer binding to distinct sites on the C3b/C4b receptor (CR1) by B D Reilly, S C Makrides, P J Ford, H C Marsh, Jr, C Mold

“…The complement receptor CR1 (CD35) is a transmembrane protein composed in its extracellular portion of short consensus repeats (SCR 1-30) organized into four…”
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Protein synthesis and degradation during aging and senescence by Makrides, S C

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Regulation of Renin Gene Expression in Hypertensive Rats by MAKRIDES, SAVVAS C, MULINARI, ROGERIO, ZANNIS, VASSILIS I, GAVRAS, HARALAMBOS

“…A carboxy terminal renin complementary DNA (cDNA) clone from rat kidney was isolated, characterized, and used as a probe for renin messenger RNA (mRNA)…”
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Components of Vectors for Gene Transfer and Expression in Mammalian Cells by Makrides, Savvas C.

“…Progress in diverse scientific fields has been realized partly by the continued refinement of mammalian gene expression vectors. A growing understanding of…”
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Spontaneous combined hyperlipidemia, coronary heart disease and decreased survival in Dahl salt-sensitive hypertensive rats transgenic for human cholesteryl ester transfer protein by Herrera, Victoria L. M, Makrides, Savvas C, Xie, Hou Xiang, Adari, Hedy, Krauss, Ronald M, Ryan, Una S, Ruiz-Opazo, Nelson

“…The acceleration of atherosclerosis by polygenic (essential) hypertension is well-characterized in humans; however, the lack of an animal model that simulates…”
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The Carboxyl-terminal Hydrophobic Residues of Apolipoprotein A-I Affect Its Rate of Phospholipid Binding and Its Association with High Density Lipoprotein by Laccotripe, Maria, Makrides, Savvas C., Jonas, Ana, Zannis, Vassilis I.

“…We performed a series of mutations in the human apolipoprotein A-I (apoA-I) gene designed to alter specific amino acid residues and domains implicated in…”
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The content and size distribution of membrane-bound and free polyribosomes in mouse liver during aging by Makrides, S C, Goldthwaite, J

“…The method of Ramsey and Steele [Anal. Biochem., 92 (1979) 305--313] was used to examine the size distribution of membrane-bound and free polyribosomes from…”
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Sequence and expression of Tangier apoA‐I gene by MAKRIDES, Savvas C., RUIZ‐OPAZO, Nelson, HAYDEN, Michael, NUSSBAUM, Alexander L., BRESLOW, Jan L., ZANNIS, Vassilis I.

“…We have isolated and characterized the apoA‐I gene from a λ L47.1 genomic library constructed with DNA obtained from the lymphocytes of a Tangier disease…”
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A tightly regulated high level expression vector that utilizes a thermosensitive lac repressor: production of the human T cell receptor V beta 5.3 in Escherichia coli by Andrews, B, Adari, H, Hannig, G, Lahue, E, Gosselin, M, Martin, S, Ahmed, A, Ford, P J, Hayman, E G, Makrides, S C

“…A series of vectors has been constructed to express the human T cell receptor V beta 5.3 under the control of the hybrid trc promoter in Escherichia coli…”
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A soluble deletion mutant of the human complement receptor type 1, which lacks the C4b binding site, is a selective inhibitor of the alternative complement pathway by Scesney, S M, Makrides, S C, Gosselin, M L, Ford, P J, Andrews, B M, Hayman, E G, Marsh, Jr, H C

“…The human complement receptor type 1 (CR1, CD35), is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus…”
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Strategies for achieving high-level expression of genes in Escherichia coli by Makrides, S C

“…Progress in our understanding of several biological processes promises to broaden the usefulness of Escherichia coli as a tool for gene expression. There is an…”
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Expression of the human T-cell receptor V beta 5.3 in Escherichia coli by thermal induction of the trc promoter: nucleotide sequence of the lacIts gene by Adari, H, Andrews, B, Ford, P J, Hannig, G, Brosius, J, Makrides, S C

“…We have constructed a vector, pKBi, for the high-level expression of the variable beta chain 5.3 (V beta 5.3) of the human T-cell receptor in Escherichia coli…”
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High level of expression of functional human platelet alpha 2-adrenergic receptors in a stable mouse C127 cell line by Bresnahan, M R, Flordellis, C S, Vassilatis, D K, Makrides, S C, Zannis, V I, Gavras, H

“…We have generated, by transfection and proper selection, a stable mouse C127 cell line which expresses the human alpha 2-adrenergic receptor gene. The size of…”
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Bioconjugation of quantum dot luminescent probes for Western blot analysis by Makrides, Savvas C, Gasbarro, Christina, Bello, Job M

“…Western blot analysis is a widely used technique for protein immunodetection. Its current format, however is unsuitable for multiplex detection of proteins,…”
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