A Comparative Analysis of Innate Immune Responses and the Structural Characterization of Spike from SARS-CoV-2 Gamma Variants and Subvariants

The SARS-CoV-2 P.1 variant, responsible for an outbreak in Manaus, Brazil, is distinguished by 12 amino acid differences in the S protein, potentially increasing its ACE-2 affinity and immune evasion capability. We investigated the innate immune response of this variant compared to the original B.1...

Full description

Saved in:

Bibliographic Details
Published in:	Microorganisms (Basel) Vol. 12; no. 4; p. 720
Main Authors:	Scovino, Aline Miranda, Dahab, Elizabeth Chen, Diniz-Lima, Israel, de Senna Silveira, Etiele, Barroso, Shana Priscila Coutinho, Cardoso, Karina Martins, Nico, Dirlei, Makhoul, Gustavo José, da Silva-Junior, Elias Barbosa, Freire-de-Lima, Celio Geraldo, Freire-de-Lima, Leonardo, Fonseca, Leonardo Marques da, Valente, Natalia, Nacife, Valeria, Machado, Ana, Araújo, Mia, Vieira, Gustavo Fioravanti, Pauvolid-Corrêa, Alex, Siqueira, Marilda, Morrot, Alexandre
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 01-04-2024
Subjects:	Affinity Amino acids Antibodies B.1 strain Comparative analysis COVID-19 COVID-19 vaccines Cytokine storm Cytokines Disease transmission Genetic aspects Genomes Health aspects Host-virus relationships Immune response Immune system Immunogenicity Infections Infectivity Innate immunity Leukocytes (mononuclear) Leukocytes (neutrophilic) mononuclear cells Multisystem inflammatory syndrome in children Mutation Neutrophils P.1 variant Proteins SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 Structural analysis Viral diseases Viral infections Virulence (Microbiology) Viruses Brazil COVID-19 mononuclear cells B.1 strain SARS-CoV-2 cytokines neutrophils P.1 variant
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The SARS-CoV-2 P.1 variant, responsible for an outbreak in Manaus, Brazil, is distinguished by 12 amino acid differences in the S protein, potentially increasing its ACE-2 affinity and immune evasion capability. We investigated the innate immune response of this variant compared to the original B.1 strain, particularly concerning cytokine production. Blood samples from three severe COVID-19 patients were analyzed post-infection with both strains. Results showed no significant difference in cytokine production of mononuclear cells and neutrophils for either variant. While B.1 had higher cytopathogenicity, neither showed viral replication in mononuclear cells. Structural analyses of the S protein highlighted physicochemical variations, which might be linked to the differences in infectivity between the strains. Our studies point to the increased infectivity of P.1 could stem from altered immunogenicity and receptor-binding affinity.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2076-2607 2076-2607
DOI:	10.3390/microorganisms12040720