Accelerating full thickness wound healing using collagen sponge of mrigal fish (Cirrhinus cirrhosus) scale origin

Accelerating full thickness wound healing using collagen sponge of mrigal fish (Cirrhinus cirrhosus) scale origin

The potentiality of collagen sponge as a skin substitute, derived from mrigal (Cirrhinus cirrhosus) scale has been explored in this study. Acid soluble collagen (ASC) and pepsin soluble collagen (PSC) from the scale of mrigal were isolated and characterized. The yields of ASC and PSC were ∼3% and ∼7...

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Bibliographic Details
Published in:International journal of biological macromolecules Vol. 93; no. Pt B; pp. 1507 - 1518
Main Authors: Pal, Pallabi, Srivas, Pavan Kumar, Dadhich, Prabhash, Das, Bodhisatwa, Maity, Priti Prasana, Moulik, Dhrubajyoti, Dhara, Santanu
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-12-2016
Subjects:
Animals
Biocompatible Materials
Cells, Cultured
Child, Preschool
Collagen - chemistry
Cyprinidae
Humans
Infant
Infant, Newborn
Male
Materials Testing
Mrigal fish scale
Prostheses and Implants
Rats, Wistar
RT-PCR
Tissue Engineering
Tissue Scaffolds - chemistry
Type I collagen
Wound Healing
Mrigal fish scale
Wound healing
Type I collagen
RT-PCR
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Summary:The potentiality of collagen sponge as a skin substitute, derived from mrigal (Cirrhinus cirrhosus) scale has been explored in this study. Acid soluble collagen (ASC) and pepsin soluble collagen (PSC) from the scale of mrigal were isolated and characterized. The yields of ASC and PSC were ∼3% and ∼7% based on the dry weight of scale while the hydroxyproline content was ∼90mg/g. Scanning electron microscope revealed progressive demineralization with EDTA on time dependent scale. Further, the D-Spacing in fibril bundles were calculated to be ∼67nm. Fourier transform infrared and circular dichroism spectra confirmed extracted protein to be collagen I, where both ASC and PSC comprised of two different α-chains (α1 and α2). The denaturation temperature (Td) of the collagen solution was 35°C closer to Td of mammalian collagen. In vitro cell culture studies on the extracted collagen sponge showed efficient cell growth and proliferation. Additionally, co-culture with fibroblast and keratinocyte cells showed development of stratified epidermal layer in vitro. Faster wound healing potential of the extracted collagen in a rat model proved its applicability as a dermal substitute.
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content type line 23
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2016.04.032