Proliferating cell nuclear antigen expression on tongue of mice after intake of, or topical exposure to, alcohol

The purpose of the current study was to evaluate the effects of alcohol on cellular proliferation. Sixty mice were separated into three groups of 20 mice in each. The first group, exposed to alcohol continuously, ingested 40% [volume/volume (vol./vol.)] alcohol instead of water during the experiment...

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Bibliographic Details
Published in:	Alcohol (Fayetteville, N.Y.) Vol. 31; no. 1; pp. 25 - 30
Main Authors:	Maito, Fábio L.D.M., Rados, Pantelis V., Filho, Manoel Sant'Ana, Barbachan, João J., Quadros, Onofre
Format:	Journal Article
Language:	English
Published:	New York, NY Elsevier Inc 01-08-2003 Elsevier Science Elsevier Limited
Subjects:	Administration, Topical Alcohol Alcohol Drinking - metabolism Alcoholism and acute alcohol poisoning Animals Biological and medical sciences Carcinogens Cell cycle Cell Division - drug effects Cell Division - physiology Cellular proliferation DNA damage Enzymes epithelial cells Ethanol Ethanol - administration & dosage Female Free radicals Gene Expression Regulation - drug effects Gene Expression Regulation - physiology Head & neck cancer Medical sciences Metabolites Mice Pathology PCNA Proliferating Cell Nuclear Antigen - analysis Proliferating Cell Nuclear Antigen - biosynthesis Proteins Rodents Studies Tongue - cytology Tongue - drug effects Tongue - metabolism Toxicology Brazil Rio Grande Alcohol PCNA Cellular proliferation Cell cycle Cell proliferation Tongue Ethanol Rodentia Oral administration Alcoholic beverage Vertebrata Mammalia Mouse Animal Cellular proliferation; Cell cycle: PCNA PCNA antigen Topical administration
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Summary:	The purpose of the current study was to evaluate the effects of alcohol on cellular proliferation. Sixty mice were separated into three groups of 20 mice in each. The first group, exposed to alcohol continuously, ingested 40% [volume/volume (vol./vol.)] alcohol instead of water during the experiment. For the second group, exposed to alcohol topically, alcohol was applied to the dorsum of the tongue twice a week. The third group served as the control group. We used the proliferating cell nuclear antigen (PCNA) immunohistochemical expression technique to perform quantitative measurements of cellular proliferation in the basal and intermediate layers of the epithelial tissue of the tongue. Cell proliferation was quantified at three different time points: just before the beginning of the experiment and at 6 and 12 months. Results were compared for mice in each group and for the three groups. At 12 months, we observed an increase in cellular proliferation in the intermediate layer of the epithelium of mice in the group that consumed alcohol ( P = .01). Results for topical alcohol–exposed and control groups did not show significant differences in cellular proliferation at any time point during the study. We concluded that the effects of alcohol on cellular proliferation may be caused by continuous intake of alcohol and occur throughout life.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0741-8329 1873-6823
DOI:	10.1016/j.alcohol.2003.06.003