Search Results - "Main, Ewan R.G."

Scalable Geometrically Designed Protein Cages Assembled via Genetically Encoded Split Inteins by Wright, James N., Wong, Wan Ling, Harvey, Joseph A., Garnett, James A., Itzhaki, Laura S., Main, Ewan R.G.

“…Engineering proteins to assemble into user-defined structures is key in their development for biotechnological applications. However, designing generic rather…”
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Repeat protein engineering: creating functional nanostructures/biomaterials from modular building blocks by Main, Ewan R G, Phillips, Jonathan J, Millership, Charlotte

“…There is enormous interest in molecular self-assembly and the development of biological systems to form smart nanostructures for biotechnology (so-called…”
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Dissecting and reprogramming the folding and assembly of tandem-repeat proteins by Rowling, Pamela J E, Sivertsson, Elin M, Perez-Riba, Albert, Main, Ewan R G, Itzhaki, Laura S

“…Studying protein folding and protein design in globular proteins presents significant challenges because of the two related features, topological complexity…”
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A high-throughput fluorescence chemical denaturation assay as a general screen for protein–ligand binding by Mahendrarajah, Kumaran, Dalby, Paul A., Wilkinson, Barrie, Jackson, Sophie E., Main, Ewan R.G.

“…Chemical denaturation of ligand–protein complexes can provide the basis of a label-free binding assay. Here, we show how the technique can be used as a…”
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Mapping the Interactions Present in the Transition State for Unfolding/Folding of FKBP12 by Fulton, Kate F, Main, Ewan R.G, Daggett, Valerie, Jackson, Sophie E

“…The structure of the transition state for folding/unfolding of the immunophilin FKBP12 has been characterised using a combination of protein engineering…”
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Exploring the “N-Terminal Anchor” Binding Interface of the T3SS Chaperone–Translocator Complexes from P. aeruginosa by Frankling, Charlotte L., Kang, Angray S., Main, Ewan R. G.

“…The type III secretion system is a large multiprotein complex that many Gram-negative bacteria use for infection. A crucial part of the complex is its…”
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Exploring the ‘N-terminal arm’ & ‘Convex surface’ Binding Interfaces of the T3SS Chaperone-Translocator Complexes from P. Aeruginosa by Frankling, Charlotte L., Downes, Megan F., Kang, Angray S., Main, Ewan R.G.

“…[Display omitted] •Major/Minor Translocator-chaperone complexes are crucial for effective T3SS operation.•Specificity of P. aeruginosa major/minor…”
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Context-Dependent Energetics of Loop Extensions in a Family of Tandem-Repeat Proteins by Perez-Riba, Albert, Lowe, Alan R., Main, Ewan R.G., Itzhaki, Laura S.

“…Consensus-designed tetratricopeptide repeat proteins are highly stable, modular proteins that are strikingly amenable to rational engineering. They therefore…”
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Decoupling a tandem-repeat protein: Impact of multiple loop insertions on a modular scaffold by Perez-Riba, Albert, Komives, Elizabeth, Main, Ewan R. G., Itzhaki, Laura S.

“…The simple topology and modular architecture of tandem-repeat proteins such as tetratricopeptide repeats (TPRs) and ankyrin repeats makes them straightforward…”
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PyFolding: Open-Source Graphing, Simulation, and Analysis of the Biophysical Properties of Proteins by Lowe, Alan R., Perez-Riba, Albert, Itzhaki, Laura S., Main, Ewan R.G.

“…For many years, curve-fitting software has been heavily utilized to fit simple models to various types of biophysical data. Although such software packages are…”
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Fibrous Nanostructures from the Self-Assembly of Designed Repeat Protein Modules by Phillips, Jonathan J., Millership, Charlotte, Main, Ewan R. G.

“…Single‐protein‐chain superhelical filaments are obtained from monomeric repeat proteins by controlling the chemistry and solvent exposure at their terminal…”
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Design of Stable α-Helical Arrays from an Idealized TPR Motif by Main, Ewan R.G., Xiong, Yong, Cocco, Melanie J., D'Andrea, Luca, Regan, Lynne

“…The tetratrico peptide repeat (TPR) is a 34-amino acid α-helical motif that occurs in over 300 different proteins. In the different proteins, three to sixteen…”
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Programmed Protein Self-Assembly Driven by Genetically Encoded Intein-Mediated Native Chemical Ligation by Harvey, Joseph A, Itzhaki, Laura S, Main, Ewan R. G

“…Harnessing and controlling self-assembly is an important step in developing proteins as novel biomaterials. With this goal, here we report the design of a…”
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Exploring the Folding Energy Landscape of a Series of Designed Consensus Tetratricopeptide Repeat Proteins by Javadi, Yalda, Main, Ewan R. G., Wolynes, Peter G.

“…Repeat proteins contain short, tandem arrays of simple structural motifs (20-40 aa). These stack together to form nonglobular structures that are stabilized by…”
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LcrH, a Class II Chaperone from the Type Three Secretion System, Has a Highly Flexible Native Structure by Singh, Sunny K., Boyle, Aimee L., Main, Ewan R.G.

“…The type three secretion system is a large and complex protein nano-machine that many Gram-negative pathogens employ to infect host cells. A key structure of…”
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A New Folding Paradigm for Repeat Proteins by Kajander, Tommi, Cortajarena, Aitziber L, Main, Ewan R. G, Mochrie, Simon G. J, Regan, Lynne

“…The folding/unfolding transitions of a series of designed consensus tetratricopeptide repeat proteins are quantitatively described by the classical…”
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A recurring theme in protein engineering: the design, stability and folding of repeat proteins by Main, Ewan RG, Lowe, Alan R, Mochrie, Simon GJ, Jackson, Sophie E, Regan, Lynne

“…Repeat proteins are ubiquitous and are involved in a myriad of essential processes. They are typically non-globular structures that act as diverse scaffolds…”
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The folding and design of repeat proteins: reaching a consensus by Main, Ewan RG, Jackson, Sophie E, Regan, Lynne

“…Although they are widely distributed across kingdoms and are involved in a myriad of essential processes, until recently, repeat proteins have received little…”
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Protein folding: Defining a “standard” set of experimental conditions and a preliminary kinetic data set of two‐state proteins by Maxwell, Karen L., Wildes, David, Zarrine‐Afsar, Arash, De Los Rios, Miguel A., Brown, Andrew G., Friel, Claire T., Hedberg, Linda, Horng, Jia‐Cherng, Bona, Diane, Miller, Erik J., Vallée‐Bélisle, Alexis, Main, Ewan R.G., Bemporad, Francesco, Qiu, Linlin, Teilum, Kaare, Vu, Ngoc‐Diep, Edwards, Aled M., Ruczinski, Ingo, Poulsen, Flemming M., Kragelund, Birthe B., Michnick, Stephen W., Chiti, Fabrizio, Bai, Yawen, Hagen, Stephen J., Serrano, Luis, Oliveberg, Mikael, Raleigh, Daniel P., Wittung‐Stafshede, Pernilla, Radford, Sheena E., Jackson, Sophie E., Sosnick, Tobin R., Marqusee, Susan, Davidson, Alan R., Plaxco, Kevin W.

“…Recent years have seen the publication of both empirical and theoretical relationships predicting the rates with which proteins fold. Our ability to test and…”
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Local and Long-Range Stability in Tandemly Arrayed Tetratricopeptide Repeats by Ewan R. G. Main, Stott, Katherine, Jackson, Sophie E., Regan, Lynne, Baldwin, Robert L.

“…The tetratricopeptide repeat (TPR) is a 34-aa α-helical motif that occurs in tandem arrays in a variety of different proteins. In natural proteins, the number…”
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