The effect of exogenous gangliosides on matrix metalloproteinase secretion by human glioma cells in vitro

Matrix metalloproteinases (MMPs) are zinc-dependent peptidases and are amongst those enzymes responsible for extracellular matrix (ECM) degradation during tumour-cell migration. Gangliosides are a family of acidic membrane glycolipids thought to play a role during cell development, differentiation a...

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Bibliographic Details
Published in:	European journal of cancer (1990) Vol. 32; no. 5; pp. 868 - 871
Main Authors:	Maidment, S.L., Merzak, A., Koochekpour, S., Rooprai, H.K., Rucklidge, G.J., Pilkington, G.J.
Format:	Journal Article
Language:	English
Published:	Oxford Elsevier Ltd 01-05-1996 Elsevier
Subjects:	Biological and medical sciences Collagenases - metabolism Culture Media, Conditioned Electrophoresis, Polyacrylamide Gel ganglioside Gangliosides - pharmacology gelatinase Gelatinases - metabolism glioma Glioma - enzymology Humans Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Medical sciences Metalloendopeptidases - metabolism metalloproteinase Neurology Tumor Cells, Cultured - drug effects Tumor Cells, Cultured - enzymology Tumors of the nervous system. Phacomatoses gelatinase glioma metalloproteinase ganglioside Human Cell culture Nervous system diseases Enzyme Pathogenesis Metalloendopeptidases In vitro Metalloproteins Gelatinase B Gelatinase A Ganglioside Glioma Central nervous system disease Hydrolases Tumor Extracellular matrix Proteinases
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Summary:	Matrix metalloproteinases (MMPs) are zinc-dependent peptidases and are amongst those enzymes responsible for extracellular matrix (ECM) degradation during tumour-cell migration. Gangliosides are a family of acidic membrane glycolipids thought to play a role during cell development, differentiation and oncogenic transformation. In this descriptive study, we investigated the effects of six exogenous gangliosides (GM1, GM3, GD1a, GD1b, GD3 and GT1b) on the secretion of MMP-2 (72 kDa gelatinase or gelatinase-A) and MMP-9 (92 kDa gelatinase or gelatinase-B). Cell-conditioned media from eight human glioma-derived cell-lines served as the source of MMPs and were investigated using SDS-PAGE zymography. Six of the cell lines showed upregulation of secretion of both enzymes by all six gangliosides. Of the remaining two cell lines, one showed inhibition of MMP secretion by all gangliosides and the other had a small but differential reponse to the range of gangliosides investigated. These results suggest that gangliosides may stimulate glioma cell invasiveness by promoting MMP expression.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0959-8049 1879-0852
DOI:	10.1016/0959-8049(95)00659-1