Proof of Concept of a Novel Multiepitope Recombinant Protein for the Serodiagnosis of Patients with Chagas Disease

Chagas disease remains a neglected disease that is considered to be a public health problem. The early diagnosis of cases is important to improve the prognosis of infected patients and prevent transmission. Serological tests are the method of choice for diagnosis. However, two serological tests are...

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Bibliographic Details
Published in:	Pathogens (Basel) Vol. 12; no. 2; p. 312
Main Authors:	Machado, Juliana Martins, Pereira, Isabela Amorim Gonçalves, Maia, Ana Clara Gontijo, Francisco, Mariana Ferraz Chaves, Nogueira, Lais Moreira, Gandra, Isadora Braga, Ribeiro, Anna Julia, Silva, Kamila Alves, Resende, Carlos Ananias Aparecido, da Silva, Jonatas Oliveira, Dos Santos, Michelli, Gonçalves, Ana Alice Maia, Tavares, Grasiele de Sousa Vieira, Chávez-Fumagalli, Miguel Angel, Campos-da-Paz, Mariana, Giunchetti, Rodolfo Cordeiro, Rocha, Manoel Otávio da Costa, Chaves, Ana Thereza, Coelho, Eduardo Antônio Ferraz, Galdino, Alexsandro Sobreira
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 01-02-2023 MDPI
Subjects:	Amino acids Antibodies Antigenic determinants Antigens Chagas disease Chronic illnesses Diagnosis E coli Enzyme-linked immunosorbent assay Enzymes Heart diseases immunodiagnosis Immunoglobulins Infections Methods multiepitope Parasites Parasitic diseases Polypeptides Proteins Public health recombinant protein Recombinant proteins Serodiagnosis Serological tests Serology Tropical diseases Trypanosoma cruzi Vector-borne diseases Brazil United States > US Chagas disease multiepitope immunodiagnosis recombinant protein
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Summary:	Chagas disease remains a neglected disease that is considered to be a public health problem. The early diagnosis of cases is important to improve the prognosis of infected patients and prevent transmission. Serological tests are the method of choice for diagnosis. However, two serological tests are currently recommended to confirm positive cases. In this sense, more sensitive and specific serological tests need to be developed to overcome these current diagnosis problems. This study aimed to develop a new recombinant multiepitope protein for the diagnosis of Chagas disease, hereafter named rTC. The rTC was constructed based on amino acid sequences from different combinations of antigens in the same polypeptide and tested using an enzyme-linked immunosorbent assay (ELISA) to detect different types of Chagas disease. rTC was able to discriminate between indeterminate (IND) and cardiac (CARD) cases and cross-reactive diseases, as well as healthy samples, with 98.28% sensitivity and 96.67% specificity, respectively. These data suggest that rTC has the potential to be tested in future studies against a larger serological panel for the diagnosis of Chagas disease.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to the work.
ISSN:	2076-0817 2076-0817
DOI:	10.3390/pathogens12020312