Search Results - "Maglott, Emily J"

Conformational Transitions of an Unmodified tRNA:  Implications for RNA Folding by Maglott, Emily J, Deo, Sanmitra S, Przykorska, Anna, Glick, Gary D

“…Unmodified tRNAs are powerful systems to study the effects of posttranscriptional modifications and site-directed mutations on both the structure and function…”
Get full text

Designed Signaling Aptamers that Transduce Molecular Recognition to Changes in Fluorescence Intensity by Jhaveri, Sulay D, Kirby, Romy, Conrad, Rick, Maglott, Emily J, Bowser, Michael, Kennedy, Robert T, Glick, Gary, Ellington, Andrew D

“…We have engineered aptamers that contain fluorescent reporters and that signal the presence of cognate ligands in solution. Two different anti-adenosine…”
Get full text

Probing the Structure of an RNA Tertiary Unfolding Transition State by Maglott, Emily J, Goodwin, Jay T, Glick, Gary D

“…The functional properties of RNA are often linked to specific tertiary structures and conformational changes that they undergo. Fully understanding RNA…”
Get full text

Probing structural elements in RNA using engineered disulfide cross-links by Maglott, E.J, Glick, G.D

“…Three analogs of unmodified yeast tRNAPhe, each possessing a single disulfide cross-link, have been designed and synthesized. One cross-link is between G1 and…”
Get full text

A new method to monitor the rate of conformational transitions in RNA by Maglott, Emily J., Glick, Gary D.

“…Many RNAs need Mg2+ to produce stable tertiary structures. Here we describe a simple method to measure the rate and activation parameters of tertiary structure…”
Get full text

Rapid magnesium chelation as a method to study real-time tertiary unfolding of RNA by Maglott, E J, Glick, G D

“…This unit describes a method to measure the unfolding of RNA tertiary structure on a millisecond time scale. A stopped-flow spectrophotometer is used to…”
Get more information

Engineering disulfide cross-links in RNA via air oxidation by Maglott, E J, Glick, G D

“…This unit presents protocols for the synthesis of alkylthiol-modified ribonucleosides, their incorporation into synthetic RNA, and the formation of…”
Get more information