Impact of Pancreatic Rat Islet Density on Cell Survival during Hypoxia

Impact of Pancreatic Rat Islet Density on Cell Survival during Hypoxia

In bioartificial pancreases (BP), the number of islets needed to restore normoglycaemia in the diabetic patient is critical. However, the confinement of a high quantity of islets in a limited space may impact islet survival, particularly in regard to the low oxygen partial pressure (PO2) in such env...

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Published in:Journal of diabetes research Vol. 2016; no. 2016; pp. 1 - 10
Main Authors: Sigrist, Séverine, Maillard, Elisa, Pinget, Michel, Mura, C., Langlois, A., Sookhareea, C., Magisson, J., Peronet, C., Bietiger, William, Rodriguez-Brotons, A., Jeandidier, Nathalie
Format: Journal Article
Language:English
Published: Cairo, Egypt Hindawi Publishing Corporation 01-01-2016
Hindawi Limited
Subjects:
Animals
Apoptosis
Atmospheric Pressure
Cell Hypoxia
Cell Survival
Cytokines - genetics
Cytokines - metabolism
Diabetes
Experiments
Gene Expression Regulation
Glucose
Hypoxia
Hypoxia-Inducible Factor 1, alpha Subunit - genetics
Hypoxia-Inducible Factor 1, alpha Subunit - metabolism
Inflammation Mediators - metabolism
Insulin
Islets of Langerhans - metabolism
Islets of Langerhans - pathology
Male
Oxygen - metabolism
Pancreas
Patients
Polymers
Rats, Wistar
Time Factors
Tissue Culture Techniques
Transplants & implants
France
United States > US
Germany
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Summary:In bioartificial pancreases (BP), the number of islets needed to restore normoglycaemia in the diabetic patient is critical. However, the confinement of a high quantity of islets in a limited space may impact islet survival, particularly in regard to the low oxygen partial pressure (PO2) in such environments. The aim of the present study was to evaluate the impact of islet number in a confined space under hypoxia on cell survival. Rat islets were seeded at three different concentrations (150, 300, and 600 Islet Equivalents (IEQ)/cm2) and cultured in normal atmospheric pressure (160 mmHg) as well as hypoxic conditions (15 mmHg) for 24 hours. Cell viability, function, hypoxia-induced changes in gene expression, and cytokine secretion were then assessed. Notably, hypoxia appeared to induce a decrease in viability and increasing islet density exacerbated the observed increase in cellular apoptosis as well as the loss of function. These changes were also associated with an increase in inflammatory gene transcription. Taken together, these data indicate that when a high number of islets are confined to a small space under hypoxia, cell viability and function are significantly impacted. Thus, in order to improve islet survival in this environment during transplantation, oxygenation is of critical importance.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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Academic Editor: Sergiu Catrina
ISSN:2314-6745
2314-6753
DOI:10.1155/2016/3615286