In vitro antiplasmodial activity, targeted LC–MS metabolite profiling, and identification of major natural products in the bioactive extracts of Palicourea and Psychotria species from the Amazonia and Atlantic Forest biomes, Brazil

Introduction A great variety of bioactive natural products has been reported for different Palicourea and Psychotria species (Rubiaceae). However, few of them as well as few of species of these botanical genera have been evaluated for antiplasmodial activity. Objective To assess the antiplasmodial a...

Full description

Saved in:

Bibliographic Details
Published in:	Metabolomics Vol. 17; no. 9; p. 81
Main Authors:	Gontijo, Douglas Costa, do Nascimento, Maria Fernanda Alves, Rody, Hugo Vianna Silva, Magalhães, Rodrigo Andrade, Margalho, Luciano Ferreira, Brandão, Geraldo Célio, de Oliveira, Alaíde Braga
Format:	Journal Article
Language:	English
Published:	New York Springer US 01-09-2021 Springer Nature B.V
Subjects:	Alkaloids Alkaloids - pharmacology Antimalarial agents Antimalarials - pharmacology Antiprotozoal agents Biochemistry Biological Products Biomedical and Life Sciences Biomedicine Brazil Cell Biology Chloroquine Chromatography, Liquid Developmental Biology Ecosystem Ethanol Forests Life Sciences Metabolites Molecular Medicine Natural products Original Article Palicourea Parasites Plant Extracts - pharmacology Plant Leaves Psychotria Rubiaceae Species Tandem Mass Spectrometry Brazil Atlantic Forest Alkaloids Bioactivity alliance Phenolics Phytochemistry Rubiaceae Psychotrieae alliance
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Introduction A great variety of bioactive natural products has been reported for different Palicourea and Psychotria species (Rubiaceae). However, few of them as well as few of species of these botanical genera have been evaluated for antiplasmodial activity. Objective To assess the antiplasmodial activity of 24 extracts from Palicourea and Psychotria genera, along with the targeted LC–MS metabolite profiling, as well as identification of the main metabolites in the bioactive extracts. Methods Twenty four ethanol and acid–base extracts from Palicourea and Psychotria genera collected in the Amazonia and Atlantic Forest, Brazil, were evaluated against chloroquine-resistant Plasmodium falciparum W2 strain by Pf LDH. The metabolite profiling and putative identification of metabolites from bioactive extracts were determined by LC-DAD-ESI–MS and LC-HRMS, respectively. Results The ethanol extracts disclosed low antiplasmodial activity (% GI < 50%). High antiplasmodial effect was observed for the acid–base extracts from Psychotria apoda and Psychotria colorata with 100% inhibition of parasite growth inhibition. Fragment ions related to pyrrolidinoindoline alkaloids were observed by LC-DAD-ESI–MS mainly in the most bioactive extracts. The results of the in vitro screening associated with the LC-DAD-ESI–MS and LC-HRMS n data allowed to predict, for the first time, the pyrrolidinoindoline alkaloids as possible antiplasmodial representing, then, new potential natural antimalarial hits. In addition, other metabolite classes such as flavanones, lignans and chalcones were also putatively identified in the bioactive extracts of Psychotria apoda, Psychotria capitata, and Psychotria poeppigiana. Conclusion The present results point to Palicourea and Psychotria species as sources of new antimalarial hits.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1573-3882 1573-3890
DOI:	10.1007/s11306-021-01833-z