Elevating serotonin pre-partum alters the Holstein dairy cow hepatic adaptation to lactation

Serotonin is known to regulate energy and calcium homeostasis in several mammalian species. The objective of this study was to determine if pre-partum infusions of 5-hydroxytryptophan (5-HTP), the immediate precursor to serotonin synthesis, could modulate energy homeostasis at the level of the hepat...

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Published in:	PloS one Vol. 12; no. 9; p. e0184939
Main Authors:	Weaver, Samantha R, Prichard, Allan S, Maerz, Noah L, Prichard, Austin P, Endres, Elizabeth L, Hernández-Castellano, Lorenzo E, Akins, Matthew S, Bruckmaier, Rupert M, Hernandez, Laura L
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 18-09-2017 Public Library of Science (PLoS)
Subjects:	Abundance Adaptation Animal lactation Animals Apoptosis Autocrine signalling Biology and Life Sciences Blood Glucose - metabolism Calcium Calcium homeostasis Caspase Caspase 3 - metabolism Caspase-3 Cattle Cyclin-dependent kinase Cyclin-dependent kinase 4 Cyclin-Dependent Kinase 4 - metabolism Dairy cattle Dairy industry Diet Energy balance Enzymes Esterification Fatty acids Fatty Acids, Nonesterified - blood Female Gene expression Glucagon Glucagon - blood Homeostasis Immunofluorescence Insulin Insulin - blood Ki-67 Antigen - metabolism Lactation Lactation - blood Liver Liver - metabolism Medicine and Health Sciences Metabolism Metabolites Milk Mitochondrial DNA Nervous system Paracrine signalling Physical Sciences Physiological aspects Physiology Postpartum Period - blood Pregnancy Proliferating cell nuclear antigen Receptor, Serotonin, 5-HT2B - biosynthesis Rodents Serotonin Serotonin - blood Urea United States United States > US Wisconsin
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Summary:	Serotonin is known to regulate energy and calcium homeostasis in several mammalian species. The objective of this study was to determine if pre-partum infusions of 5-hydroxytryptophan (5-HTP), the immediate precursor to serotonin synthesis, could modulate energy homeostasis at the level of the hepatocyte in post-partum Holstein and Jersey dairy cows. Twelve multiparous Holstein cows and twelve multiparous Jersey cows were intravenously infused daily for approximately 7 d pre-partum with either saline or 1 mg/kg bodyweight of 5-HTP. Blood was collected for 14 d post-partum and on d30 post-partum. Liver biopsies were taken on d1 and d7 post-partum. There were no changes in the circulating concentrations of glucose, insulin, glucagon, non-esterified fatty acids, or urea nitrogen in response to treatment, although there were decreased beta-hydroxybutyrate concentrations with 5-HTP treatment around d6 to d10 post-partum, particularly in Jersey cows. Cows infused with 5-HTP had increased hepatic serotonin content and increased mRNA expression of the serotonin 2B receptor on d1 and d7 post-partum. Minimal changes were seen in the hepatic mRNA expression of various gluconeogenic enzymes. There were no changes in the mRNA expression profile of cell-cycle progression marker cyclin-dependent kinase 4 or apoptotic marker caspase 3, although proliferating cell nuclear antigen expression tended to be increased in Holstein cows infused with 5-HTP on d1 post-partum. Immunofluorescence assays showed an increased number of CASP3- and Ki67-positive cells in Holstein cows infused with 5-HTP on d1 post-partum. Given the elevated hepatic serotonin content and increased mRNA abundance of 5HTR2B, 5-HTP infusions may be stimulating an autocrine-paracrine adaptation to lactation in the Holstein cow liver.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Competing Interests: The authors have declared that no competing interests exist.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0184939