Structural and functional analysis of the newt lymphatic system

Structural and functional analysis of the newt lymphatic system

Regeneration competent vertebrates such as newts and salamanders possess a weakened adaptive immune system characterized by multiple connections between the lymphatic system and the blood vascular system called lymphatic hearts. The role of lymphatic vasculature and these lymphaticovenous connection...

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Published in:Scientific reports Vol. 13; no. 1; p. 6902
Main Authors: Banda, Chihena H., Shiraishi, Makoto, Mitsui, Kohei, Okada, Yoshimoto, Danno, Kanako, Ishiura, Ryohei, Maemura, Kaho, Chiba, Chikafumi, Mizoguchi, Akira, Imanaka-Yoshida, Kyoko, Maruyama, Kazuaki, Narushima, Mitsunaga
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 27-04-2023
Nature Publishing Group
Nature Portfolio
Subjects:
631/136/290
631/1647/245
631/250/1617
631/250/2503
631/532/489
631/601/1737
Animals
Bone marrow
Cardiovascular System
Computed tomography
Cynops pyrrhogaster
Heart
Humanities and Social Sciences
Immune system
Lymphangiography
Lymphatic drainage
Lymphatic System
Lymphatic Vessels - diagnostic imaging
Lymphatic Vessels - metabolism
Mammals
multidisciplinary
Regeneration
Salamandridae
Science
Science (multidisciplinary)
Structure-function relationships
Vascular endothelial growth factor receptors
Vascular system
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Summary:Regeneration competent vertebrates such as newts and salamanders possess a weakened adaptive immune system characterized by multiple connections between the lymphatic system and the blood vascular system called lymphatic hearts. The role of lymphatic vasculature and these lymphaticovenous connections in regeneration is unknown. We used in-vivo near-infrared lymphangiography, ultra-high frequency ultrasonography, micro-CT lymphangiography, and histological serial section 3-dimentional computer reconstruction to evaluate the lymphatic territories of Cynops pyrrhogaster . We used our model and supermicrosurgery to show that lymphatic hearts are not essential for lymphatic circulation and limb regeneration. Instead, newts possess a novel intraosseous network of lymphatics inside the bone expressing VEGFR-3, LYVE-1 and CD-31. However, we were unable to show Prox-1 expression by these vessels. We demonstrate that adult newt bone marrow functions as both a lymphatic drainage organ and fat reservoir. This study reveals the fundamental anatomical differences between the immune system of urodeles and mammals and provides a model for investigating lymphatics and regeneration.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-34169-w