Isolation of marine hydrocarbonoclastic bacteria from petroleum contaminated sites in Dumai

The Dumai Sea is vulnerable to pollution, especially oil spills. Oil pollution in Dumai seawater causes a decline in the population of aquatic organisms. Isolation and screening of marine hydrocarbonoclastic bacteria is one effort to find potential local agents. This research aims to isolate marine...

Full description

Saved in:

Bibliographic Details
Published in:	BIO web of conferences Vol. 74; p. 4005
Main Authors:	Mardhiah Batubara, Ummi, D’rita Sibagariang, Rina, Fatmawati, Riska, Ersyi Darfia, Novreta, Yahya Ginting, Topan, Maelina, Teti
Format:	Journal Article
Language:	English
Published:	EDP Sciences 01-01-2023
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The Dumai Sea is vulnerable to pollution, especially oil spills. Oil pollution in Dumai seawater causes a decline in the population of aquatic organisms. Isolation and screening of marine hydrocarbonoclastic bacteria is one effort to find potential local agents. This research aims to isolate marine hydrocarbonoclastic bacteria from the Petroleum Contaminated Site in Dumai. This research uses a survey method, and it was in four stages such as, isolation, screening, morphological and physiological characteristics, bacterial identification, and data analysis. Water samples were taken using purposive sampling at seven different locations. The samples were analyzed in situ, including water characteristics, pH, temperature, salinity, and brightness. Furthermore, the water samples were further analyzed at the Marine Microbiology Laboratory, University of Riau. Isolation and screening results showed that ten isolates of marine hydrocarbonoclastic bacteria can grow in SSMS liquid media with a petroleum content of 5%. The ten isolates in sequence are DS21a, DS22b, DS42c, DS52d, DS61e, DS62f, DS63g, DS71h, DS72i and DS73j. The characterization and identification results obtained four different genera from petroleum-contaminated sites in Dumai, sequentially Micrococcus, Rhodococcus, Marinobacter , and Bacillus .
ISSN:	2117-4458 2117-4458
DOI:	10.1051/bioconf/20237404005