Suppressive Effect of Juzen-Taiho-To on Lung Metastasis of B16 Melanoma Cells In Vivo

Suppressive Effect of Juzen-Taiho-To on Lung Metastasis of B16 Melanoma Cells In Vivo

Juzen-Taiho-To (JTT) is well known to be one of Kampo (Japanese herbal) medicine consisted of 10 component herbs and used for the supplemental therapy of cancer patients with remarkably success. However, the precise mechanisms by which JTT could favorably modify the clinical conditions of cancer pat...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine Vol. 2011; no. 2011; pp. 1 - 5
Main Authors: Matsuda, Takako, Maekawa, Katsuhiko, Asano, Kazuhito, Hisamitsu, Tadashi
Format: Journal Article
Language:English
Published: Cairo, Egypt Hindawi Puplishing Corporation 01-01-2011
Hindawi Publishing Corporation
Hindawi Limited
Subjects:
Cancer
Cancer therapies
Cell activation
Chemotherapy
Cytokines
Drug dosages
Experiments
Health surveillance
Herbal medicine
Immunology
Inhibition
Injection
Interleukin 12
Intravenous administration
Lung cancer
Lungs
Lymphocytes T
Medicine
Melanoma
Metastases
Metastasis
Mice
Monoclonal antibodies
Natural killer cells
Oral administration
Original
Pharmaceuticals
Radiation therapy
T cell receptors
γ-Interferon
Japan
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Summary:Juzen-Taiho-To (JTT) is well known to be one of Kampo (Japanese herbal) medicine consisted of 10 component herbs and used for the supplemental therapy of cancer patients with remarkably success. However, the precise mechanisms by which JTT could favorably modify the clinical conditions of cancer patients are not well defined. The present study, therefore, was undertaken to examine the possible mechanisms of JTT on prevention of cancer metastasis using experimental mouse model. JTT was well mixed with rodent chow at concentrations of either 0.2 or 1.0%, and administered orally ad libitum, which was started 1 week before tumor cell injection and continue throughout the experiment. Oral administration of JTT at concentration 0.2 and 1.0% into C57BL/6 male mice significantly inhibited tumor metastasis in lungs, which was induced by the intravenous injection of 2 × 105 B16 melanoma cell. JTT at a concentration of 1.0% also significantly suppressed lung metastasis of B16 melanoma cell from hind footpad in C57BL/6 mice. In the second part of experiments, the influence of the depression of natural killer (NK) cell, natural killer T (NKT) cell and several types of cytokines on JTT-mediated inhibition of tumor cell metastasis. Intraperitoneal injection of anti asialo-GM1 antibody against NK cells and anti NK-1.1 monoclonal antibody (mAb) to NKT cells abrogated the inhibitory action of JTT on lung metastasis of B16 melanoma cells. Although intraperitoneal administration of anti-IFN-γ mAb scarcely affected the inhibitory action of JTT on tumor cell metastasis, injection of amrinone, which used for IL-12 suppression, significantly decreased the ability of JTT to prevent tumor cell metastasis. These results strongly suggest that oral administration of JTT caused increase in the production of IL-12, which is responsible for the activation of both NK cell and NKT cell, in the lungs and results in inhibition of B16 melanoma cell metastasis in the lungs.
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ISSN:1741-427X
1741-4288
DOI:10.1093/ecam/nen081