Search Results - "Madsen, James"

In vivo CRISPR base editing of PCSK9 durably lowers cholesterol in primates by Musunuru, Kiran, Chadwick, Alexandra C., Mizoguchi, Taiji, Garcia, Sara P., DeNizio, Jamie E., Reiss, Caroline W., Wang, Kui, Iyer, Sowmya, Dutta, Chaitali, Clendaniel, Victoria, Amaonye, Michael, Beach, Aaron, Berth, Kathleen, Biswas, Souvik, Braun, Maurine C., Chen, Huei-Mei, Colace, Thomas V., Ganey, John D., Gangopadhyay, Soumyashree A., Garrity, Ryan, Kasiewicz, Lisa N., Lavoie, Jennifer, Madsen, James A., Matsumoto, Yuri, Mazzola, Anne Marie, Nasrullah, Yusuf S., Nneji, Joseph, Ren, Huilan, Sanjeev, Athul, Shay, Madeleine, Stahley, Mary R., Fan, Steven H. Y., Tam, Ying K., Gaudelli, Nicole M., Ciaramella, Giuseppe, Stolz, Leslie E., Malyala, Padma, Cheng, Christopher J., Rajeev, Kallanthottathil G., Rohde, Ellen, Bellinger, Andrew M., Kathiresan, Sekar

“…Gene-editing technologies, which include the CRISPR–Cas nucleases 1 – 3 and CRISPR base editors 4 , 5 , have the potential to permanently modify…”
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Amino acid addition to Vibrio cholerae LPS establishes a link between surface remodeling in Gram-positive and Gram-negative bacteria by Hankins, Jessica V, Madsen, James A, Giles, David K, Brodbelt, Jennifer S, Trent, M. Stephen

“…Historically, the O1 El Tor and classical biotypes of Vibrio cholerae have been differentiated by their resistance to the antimicrobial peptide polymyxin B…”
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Ultrafast Ultraviolet Photodissociation at 193 nm and its Applicability to Proteomic Workflows by Madsen, James A, Boutz, Daniel R, Brodbelt, Jennifer S

“…Ultraviolet photodissociation (UVPD) at 193 nm was implemented on a linear ion trap mass spectrometer for high-throughput proteomic workflows. Upon irradiation…”
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Concurrent Automated Sequencing of the Glycan and Peptide Portions of O‑Linked Glycopeptide Anions by Ultraviolet Photodissociation Mass Spectrometry by Madsen, James A, Ko, Byoung Joon, Xu, Hua, Iwashkiw, Jeremy A, Robotham, Scott A, Shaw, Jared B, Feldman, Mario F, Brodbelt, Jennifer S

“…O-Glycopeptides are often acidic owing to the frequent occurrence of acidic saccharides in the glycan, rendering traditional proteomic workflows that rely on…”
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Data-independent oxonium ion profiling of multi-glycosylated biotherapeutics by Madsen, James A, Farutin, Victor, Lin, Yin Yin, Smith, Stephen, Capila, Ishan

“…The characterization of glycosylation is required for many protein therapeutics. The emergence of antibody and antibody-like molecules with multiple glycan…”
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Toward the complete characterization of host cell proteins in biotherapeutics via affinity depletions, LC-MS/MS, and multivariate analysis by Madsen, James A, Farutin, Victor, Carbeau, Theresa, Wudyka, Steve, Yin, Yan, Smith, Stephen, Anderson, James, Capila, Ishan

“…Host cell protein (HCP) impurities are generated by the host organism during the production of therapeutic recombinant proteins, and are difficult to remove…”
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193-nm photodissociation of singly and multiply charged peptide anions for acidic proteome characterization by Madsen, James A., Kaoud, Tamer S., Dalby, Kevin N., Brodbelt, Jennifer S.

“…193‐nm ultraviolet photodissociation (UVPD) was implemented to sequence singly and multiply charged peptide anions. Upon dissociation by this method,…”
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Elucidation of a novel Vibrio cholerae lipid A secondary hydroxy‐acyltransferase and its role in innate immune recognition by Hankins, Jessica V., Madsen, James A., Giles, David K., Childers, Brandon M., Klose, Karl E., Brodbelt, Jennifer S., Trent, M. Stephen

“…Summary Similar to most Gram‐negative bacteria, the outer leaflet of the outer membrane of Vibrio cholerae is comprised of lipopolysaccharide. Previous reports…”
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Infrared Photoactivation Reduces Peptide Folding and Hydrogen-Atom Migration following ETD Tandem Mass Spectrometry by Ledvina, Aaron R, McAlister, Graeme C, Gardner, Myles W, Smith, Suncerae I, Madsen, James A, Schwartz, Jae C, Stafford, George C. Jr, Syka, John E.P, Brodbelt, Jennifer S, Coon, Joshua J

“…Peptides see the light: The photoactivation of peptide precursor cations during electron transfer dissociation (ETD) led to the generation of isotopic cluster…”
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Spacer Fidelity Assessments of Guide RNA by Top-Down Mass Spectrometry by Macias, Luis A., Garcia, Sara P., Back, Kayla M., Wu, Yue, Johnson, G. Hall, Kathiresan, Sekar, Bellinger, Andrew M., Rohde, Ellen, Freitas, Michael A., Madsen, James A.

“…The advancement of CRISPR-based gene editing tools into biotherapeutics offers the potential for cures to genetic disorders and for new treatment paradigms for…”
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IR and UV Photodissociation as Analytical Tools for Characterizing Lipid A Structures by Madsen, James A, Cullen, Thomas W, Trent, M. Stephen, Brodbelt, Jennifer S

“…The utility of 193-nm ultraviolet photodissociation (UVPD) and 10.6-μm infrared multiphoton dissociation (IRMPD) for the characterization of lipid A structures…”
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Covalent Labeling Denaturation Mass Spectrometry for Sensitive Localized Higher Order Structure Comparisons by Madsen, James A, Yin, Yan, Qiao, Jing, Gill, Vanessa, Renganathan, Kutralanathan, Fu, Wing-Yee, Smith, Stephen, Anderson, James

“…Protein higher order structure (HOS) describes the three-dimensional folding arrangement of a given protein and plays critical roles in structure/function…”
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Simplifying Fragmentation Patterns of Multiply Charged Peptides by N-Terminal Derivatization and Electron Transfer Collision Activated Dissociation by Madsen, James A, Brodbelt, Jennifer S

“…N-terminal peptide derivatization strategies used in conjunction with tandem mass spectrometry to yield simplified fragmentation patterns have shown limited…”
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Tyrosine sulfation in a Gram-negative bacterium by Han, Sang-Wook, Lee, Sang-Won, Bahar, Ofir, Schwessinger, Benjamin, Robinson, Michelle R., Shaw, Jared B., Madsen, James A., Brodbelt, Jennifer S., Ronald, Pamela C.

“…Tyrosine sulfation, a well-characterized post-translation modification in eukaryotes, has not previously been reported in prokaryotes. Here, we demonstrate…”
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Characterization of Unique Modification of Flagellar Rod Protein FlgG by Campylobacter jejuni Lipid A Phosphoethanolamine Transferase, Linking Bacterial Locomotion and Antimicrobial Peptide Resistance by Cullen, Thomas W., Madsen, James A., Ivanov, Petko L., Brodbelt, Jennifer S., Trent, M. Stephen

“…Gram-negative bacteria assemble complex surface structures that interface with the surrounding environment and are involved in pathogenesis. Recent work in…”
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Systematic Comparison of Ultraviolet Photodissociation and Electron Transfer Dissociation for Peptide Anion Characterization by Shaw, Jared B., Madsen, James A., Xu, Hua, Brodbelt, Jennifer S.

“…Ultraviolet photodissociation at 193 nm (UVPD) and negative electron transfer dissociation (NETD) were compared to establish their utility for characterizing…”
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The outer membrane of Gram-negative bacteria: lipid A isolation and characterization by Hankins, Jessica V, Madsen, James A, Needham, Brittany D, Brodbelt, Jennifer S, Trent, M Stephen

“…The isolation and characterization of the lipid A domain of lipopolysaccharide (LPS) are important methodologies utilized to gain understanding of the…”
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Comparison of Infrared Multiphoton Dissociation and Collision-Induced Dissociation of Supercharged Peptides in Ion Traps by Madsen, James A., Brodbelt, Jennifer S.

“…The number and types of diagnostic ions obtained by infrared multiphoton dissociation (IRMPD) and collision-induced dissociation (CID) were evaluated for…”
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High-throughput Database Search and Large-scale Negative Polarity Liquid Chromatography–Tandem Mass Spectrometry with Ultraviolet Photodissociation for Complex Proteomic Samples by Madsen, James A., Xu, Hua, Robinson, Michelle R., Horton, Andrew P., Shaw, Jared B., Giles, David K., Kaoud, Tamer S., Dalby, Kevin N., Trent, M. Stephen, Brodbelt, Jennifer S.

“…The use of ultraviolet photodissociation (UVPD) for the activation and dissociation of peptide anions is evaluated for broader coverage of the proteome. To…”
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193 nm Ultraviolet Photodissociation of Imidazolinylated Lys-N Peptides for De Novo Sequencing by Robinson, Michelle R, Madsen, James A, Brodbelt, Jennifer S

“…The goal of many MS/MS de novo sequencing strategies is to generate a single product ion series that can be used to determine the precursor ion sequence. Most…”
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