Detection of antibodies against porcine parvovirus nonstructural protein NS1 may distinguish between vaccinated and infected pigs

The humoral antibody response against the nonstructural protein NS1 and the structural protein VP2 of porcine parvovirus (PPV) was evaluated by immuno-peroxidase test (IPT) and enzyme linked immuno sorbent assay (ELISA) using recombinant PPV antigens. The coding sequence for NS1 and VP2 was inserted...

Full description

Saved in:

Bibliographic Details
Published in:	Veterinary microbiology Vol. 54; no. 1; pp. 1 - 16
Main Authors:	Madsen, Eva Smedegaard, Madsen, Knud Gert, Nielsen, Jens, Jensen, Merethe Holm, Lei, Jørgen Christian, Have, Per
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 1997 Elsevier Science
Subjects:	Animals ANTIBODIES Antibodies, Viral - blood Antibody Formation ANTICORPS ANTICUERPOS Baculovirus Base Sequence Biological and medical sciences CERDO DNA Primers Enzyme-Linked Immunosorbent Assay Experimental viral diseases and models Fundamental and applied biological sciences. Psychology IMMUNE RESPONSE Immunoenzyme Techniques INFECCION INFECTION Infectious diseases Medical sciences Microbiology Nonstructural proteins Parvoviridae Infections - immunology Parvoviridae Infections - prevention & control Parvoviridae Infections - veterinary Parvovirus - immunology Parvovirus - isolation & purification PARVOVIRUS PORCIN PARVOVIRUS PORCINO Polymerase Chain Reaction PORCIN PORCINE PARVOVIRUS PROTEINAS PROTEINE PROTEINS Recombinant Proteins - biosynthesis Recombinant Proteins - immunology REPONSE IMMUNITAIRE RESPUESTA INMUNOLOGICA Spodoptera SWINE Swine Diseases Transfection VACCINATION Vaccination - veterinary Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies VACUNACION Viral diseases Viral Nonstructural Proteins - biosynthesis Viral Nonstructural Proteins - immunology Viral Vaccines Virology Nonstructural proteins Baculovirus Vaccination Porcine parvovirus Parvovirus Antibody Serology Veterinary medicine Differential diagnostic Immunoperoxidase technique Immunological method Pig Infection Virus Vertebrata Mammalia Parvoviridae Viral disease Artiodactyla ELISA assay Ungulata Parvovirinae
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The humoral antibody response against the nonstructural protein NS1 and the structural protein VP2 of porcine parvovirus (PPV) was evaluated by immuno-peroxidase test (IPT) and enzyme linked immuno sorbent assay (ELISA) using recombinant PPV antigens. The coding sequence for NS1 and VP2 was inserted into the baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) genome resulting in two recombinant baculoviruses AcNPV-NS1 and AcNPV-VP2, respectively. Sf9 cells ( Spodoptora frugidiperda) inoculated with AcNPV-NS1 producing recombinant nonstructural protein (rNS1) and AcNPV-VP2 producing recombinant virion protein (rVP2) were used in IPT and ELISA to analyse serum antibodies. Pigs vaccinated with an inactivated whole virus vaccine and experimentally infected pigs were studied. Significant titers against rVP2 were obtained in both vaccinated and infected pigs. Specific antibodies against rNS1 could only be detected in infected pigs and NS1 may in this way allow the specific detection of infected animals. Analysis of serum samples collected up to 18 days post infection (p.i.) from four pigs experimentally infected with PPV showed that antibodies against rNS1 and rVP2 could in all cases be detected on day 9 p.i. Two individual pigs were inoculated twice with PPV and the antibody response was followed 89 days after second inoculation. Serum antibodies against borth rVP2 and rNS1 could be detected for this period of time.
Bibliography:	L73 1998000001 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0378-1135 1873-2542
DOI:	10.1016/S0378-1135(96)01270-9