Rapid thymectomy of NSG mice to analyze the role of native and grafted thymi in humanized mice

Rapid thymectomy of NSG mice to analyze the role of native and grafted thymi in humanized mice

We developed a rapid method to remove the native mouse thymus from NSG mice, which allowed us to compare the behavior of human immune cells in the presence or absence of human T cells in human immune system mice. Removing the native mouse thymus is critical for studies of human thymopiesis in grafte...

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Bibliographic Details
Published in:European journal of immunology Vol. 50; no. 1; pp. 138 - 141
Main Authors: Khosravi‐Maharlooei, Mohsen, Hoelzl, Markus, Li, Hao Wei, Madley, Rachel C., Waffarn, Elizabeth E., Danzl, Nichole M., Sykes, Megan
Format: Journal Article
Language:English
Published: Germany Wiley Subscription Services, Inc 01-01-2020
Subjects:
Animal tissues
Animals
Hematopoietic stem cells
Human behavior
Humanized mice
Humans
Immune system
Lymphocytes
Lymphocytes T
Mice
Mice, Inbred NOD
Mice, SCID
NSG mice
Thymectomy
Thymectomy - methods
Thymopoiesis
Thymus
Thymus Gland - immunology
Thymus Gland - transplantation
Transplantation, Heterologous - methods
Thymopoiesis
NSG mice
Thymectomy
Humanized mice
Hematopoietic stem cells
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Summary:We developed a rapid method to remove the native mouse thymus from NSG mice, which allowed us to compare the behavior of human immune cells in the presence or absence of human T cells in human immune system mice. Removing the native mouse thymus is critical for studies of human thymopiesis in grafted thymic tissue in humanized mice.
Bibliography:https://publons.com/publon/10.1002/eji.201948205
The peer review history for this article is available at
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-2980
1521-4141
DOI:10.1002/eji.201948205