Aqueous seed extract of Syzygium cumini inhibits the dipeptidyl peptidase IV and adenosine deaminase activities, but it does not change the CD26 expression in lymphocytes in vitro

Syzygium cumini (Sc) have been intensively studied in the last years due its beneficial effects including anti-diabetic and anti-inflammatory potential. Thus, the aim of this study was to evaluate the effect of aqueous seed extract of Sc (ASc) in the activity of enzymes involved in lymphocyte functi...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of physiology and biochemistry Vol. 69; no. 1; pp. 119 - 124
Main Authors:	Bellé, Luziane Potrich, Bitencourt, Paula Eliete Rodrigues, Abdalla, Faida Husein, Santos de Bona, Karine, Peres, Alessandra, Maders, Liési Diones Konzen, Moretto, Maria Beatriz
Format:	Journal Article
Language:	English
Published:	Dordrecht Springer Netherlands 01-03-2013
Subjects:	Acetylcholinesterase - metabolism Adenosine Deaminase - metabolism Adenosine Deaminase Inhibitors - pharmacology Adult Animal Physiology Biomedical and Life Sciences Biomedicine Cell Survival - drug effects Cells, Cultured Dipeptidyl Peptidase 4 - genetics Dipeptidyl Peptidase 4 - metabolism Dipeptidyl-Peptidase IV Inhibitors - pharmacology Dose-Response Relationship, Drug Female Gene Expression - drug effects Human Physiology Humans Lymphocytes - cytology Lymphocytes - drug effects Lymphocytes - metabolism Male Original Paper Plant Extracts - isolation & purification Plant Extracts - pharmacology Seeds - chemistry Signal Transduction - drug effects Syzygium - chemistry Dipeptidyl peptidase IV Adenosine deaminase Immunomodulatory Lymphocytes
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Syzygium cumini (Sc) have been intensively studied in the last years due its beneficial effects including anti-diabetic and anti-inflammatory potential. Thus, the aim of this study was to evaluate the effect of aqueous seed extract of Sc (ASc) in the activity of enzymes involved in lymphocyte functions. To perform this study, we isolated lymphocytes from healthy donors. Lymphocytes were exposed to 10, 30, and 100 mg/mL of ASc during 4 and 6 h and adenosine deaminase (ADA), dipeptidyl peptidase IV (DPP-IV), and acetylcholinesterase (AChE) activities as well as CD26 expression and cellular viability were evaluated. ASc inhibited the ADA and DPP-IV activities without alteration in the CD26 expression (DPP-IV protein). No alterations were observed in the AChE activity or in the cell viability. These results indicate that the inhibition of the DPP-IV and ADA activities was dependent on the time of exposition to ASc. We suggest that ASc exhibits immunomodulatory properties probably via the pathway of DPP-IV–ADA complex, contributing to the understanding of these proceedings in the purinergic signaling.
ISSN:	1138-7548 1877-8755
DOI:	10.1007/s13105-012-0195-6