Search Results - "Madden, Knut R."

Increased Gene Targeting in Ku70 and Xrcc4 Transiently Deficient Human Somatic Cells by Bertolini, Luciana R, Bertolini, Marcelo, Maga, Elizabeth A, Madden, Knut R, Murray, James D

“…The insertion of foreign DNA at a specific genomic locus directed by homologous DNA sequences, or gene targeting, is an inefficient process in mammalian…”
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SILAC Compatible Strain of Pichia pastoris for Expression of Isotopically Labeled Protein Standards and Quantitative Proteomics by Austin, Ryan J, Kuestner, Rolf E, Chang, Debbie K, Madden, Knut R, Martin, Daniel B

“…The methylotrophic yeast Pichia pastoris is a powerful eukaryotic platform for the production of heterologous protein. Recent publication of the P. pastoris…”
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DNA topoisomerase I is involved in both repression and activation of transcription by Merino, Alejandro, Madden, Knut R, Lane, William S, Champoux, James J, Reinberg, Danny

“…Reconstituted transcription reactions containing the seven general transcription factors, in addition to RNA polymerase II, respond poorly to transcriptional…”
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Transient depletion of Ku70 and Xrcc4 by RNAi as a means to manipulate the non-homologous end-joining pathway by Bertolini, Luciana R., Bertolini, Marcelo, Anderson, Gary B., Maga, Elizabeth A., Madden, Knut R., Murray, James D.

“…Non-homologous end joining (NHEJ) is the major DNA double-strand break (DSB) repair pathway in mammalian cells and is likely responsible for the non-homologous…”
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Biochemical and Biophysical Analyses of Recombinant Forms of Human Topoisomerase I (∗) by Stewart, Lance, Ireton, Gregory C., Parker, Leon H., Madden, Knut R., Champoux, James J.

“…Amino acid sequence comparisons of human topoisomerase I (Topo I) with seven other cellular Topo I enzymes reveal that the enzyme can be divided into four…”
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Overexpression of human topoisomerase I in baby hamster kidney cells : hypersensitivity of clonal isolates to camptothecin by MADDEN, K. R, CHAMPOUX, J. J

“…The 3645-base pair human topoisomerase I complementary DNA (cDNA) clone isolated by D'Arpa et al. (Proc. Natl. Acad. Sci. USA, 85:2543-2547, 1988) and a…”
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Use of site-specific recombination to regenerate selectable markers by Cregg, J M, Madden, K R

“…A method which allows the repeated use of a single selectable marker in DNA transformations was demonstrated. This marker regeneration method employed portions…”
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Pichia pastoris as a Host System for Transformations by Cregg, James M., Barringer, Kevin J., Hessler, Anita Y., Madden, Knut R.

“…We developed a methylotrophic yeast, Pichia pastoris, as a host for DNA transformations. The system is based on an auxotrophic mutant host of P. pastoris which…”
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Preferential binding of human topoisomerase I to superhelical DNA by Madden, K. R., Stewart, L., Champoux, J. J.

“…Eukaryotic type I DNA topoisomerase provides swivels for removing torsional strain from the DNA helix during transcription and DNA replication. Previously it…”
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Expression of wild type and mutant human topoisomerase IcDNAs and use of the mutant protein to characterize DNA binding as a function of DNA topology by Madden, Knut R

“…Eukaryotic topoisomerase I (topo I) facilitates the topological changes in DNA that result from transcription, replication and recombination by catalyzing the…”
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Functional Characterization of the Two Alcohol Oxidase Genes from the Yeast Pichia pastoris by Cregg, James M., Madden, Knut R., Barringer, Kevin J., Thill, Gregory P., Stillman, Cathy A.

“…In Pichia pastoris, alcohol oxidase (AOX) is the first enzyme in the methanol utilization pathway and is encoded by two genes, AOX1 and AOX2. The DNA and…”
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