An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains

To select a Saccharomyces cerevisiae reference strain amenable to experimental techniques used in (molecular) genetic, physiological and biochemical engineering research, a variety of properties were studied in four diploid, prototrophic laboratory strains. The following parameters were investigated...

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Published in:Enzyme and microbial technology Vol. 26; no. 9-10; pp. 706 - 714
Main Authors: van Dijken, J.P, Bauer, J, Brambilla, L, Duboc, P, Francois, J.M, Gancedo, C, Giuseppin, M.L.F, Heijnen, J.J, Hoare, M, Lange, H.C, Madden, E.A, Niederberger, P, Nielsen, J, Parrou, J.L, Petit, T, Porro, D, Reuss, M, van Riel, N, Rizzi, M, Steensma, H.Y, Verrips, C.T, Vindeløv, J, Pronk, J.T
Format: Journal Article Conference Proceeding
Language:English
Published: Amsterdam Elsevier Inc 01-06-2000
Elsevier Science
Elsevier
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Summary:To select a Saccharomyces cerevisiae reference strain amenable to experimental techniques used in (molecular) genetic, physiological and biochemical engineering research, a variety of properties were studied in four diploid, prototrophic laboratory strains. The following parameters were investigated: 1) maximum specific growth rate in shake-flask cultures; 2) biomass yields on glucose during growth on defined media in batch cultures and steady-state chemostat cultures under controlled conditions with respect to pH and dissolved oxygen concentration; 3) the critical specific growth rate above which aerobic fermentation becomes apparent in glucose-limited accelerostat cultures; 4) sporulation and mating efficiency; and 5) transformation efficiency via the lithium-acetate, bicine, and electroporation methods. On the basis of physiological as well as genetic properties, strains from the CEN.PK family were selected as a platform for cell-factory research on the stoichiometry and kinetics of growth and product formation.
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ISSN:0141-0229
1879-0909
DOI:10.1016/S0141-0229(00)00162-9