WtsE, an AvrE-Family Effector Protein from Pantoea stewartii subsp. stewartii, Causes Disease-Associated Cell Death in Corn and Requires a Chaperone Protein for Stability

The pathogenicity of Pantoea stewartii subsp. stewartii to sweet corn and maize requires a Hrp type III secretion system. In this study, we genetically and functionally characterized a disease-specific (Dsp) effector locus, composed of wtsE and wtsF, that is adjacent to the hrp gene cluster. WtsE, a...

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Bibliographic Details
Published in:	Molecular plant-microbe interactions Vol. 19; no. 10; pp. 1092 - 1102
Main Authors:	Ham, J.H, Majerczak, D.R, Arroyo-Rodriguez, A.S, Mackey, D.M, Coplin, D.L
Format:	Journal Article
Language:	English
Published:	St Paul, MN APS Press 01-10-2006 The American Phytopathological Society
Subjects:	Amino Acid Motifs Apoptosis bacterial diseases of plants Bacterial plant pathogens bacterial proteins Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - physiology Biological and medical sciences Chromosome Mapping corn cycloheximide Cycloheximide - pharmacology DspB/F effector proteins Erwinia amylovora Erwinia chrysanthemi Erwinia stewartii Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology genes genetic complementation Genetic Complementation Test hypersensitive response loci microbial genetics molecular chaperones Molecular Chaperones - physiology Molecular Sequence Data Multigene Family Operon Pantoea - genetics Pantoea - metabolism Pantoea - pathogenicity Pantoea stewartii Pantoea stewartii subsp. stewartii pathogenicity Pectobacterium chrysanthemi Pectobacterium chrysanthemi - genetics Phytopathology. Animal pests. Plant and forest protection Plant Diseases - microbiology Plant Leaves - cytology Plant Leaves - drug effects Plant Leaves - microbiology Type III secretion system virulence Zea mays Zea mays - cytology Zea mays - drug effects Zea mays - microbiology Monocotyledones Plant pathogen Pathogenesis Escherichia coli Complement Type III secretion system Cereal crop Pantoea stewartii Gramineae Angiospermae Bacteria Erwinia stewartii Enterobacteriaceae Gene cluster Zea mays Chaperone Zea mays var. saccharata Pesticides DspB/F Erwinia amylovora Fungicide Protein Strain Pathogenicity Cycloheximide Antibiotic Vegetable crop Cell death Erwinia chrysanthemi Spermatophyta Endoplasmic reticulum
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Summary:	The pathogenicity of Pantoea stewartii subsp. stewartii to sweet corn and maize requires a Hrp type III secretion system. In this study, we genetically and functionally characterized a disease-specific (Dsp) effector locus, composed of wtsE and wtsF, that is adjacent to the hrp gene cluster. WtsE, a member of the AvrE family of effector proteins, was essential for pathogenesis on corn and was complemented by DspA/E from Erwinia amylovora. An intact C-terminus of WtsE, which contained a putative endoplasmic reticulum membrane retention signal, was important for function of WtsE. Delivery of WtsE into sweet corn leaves by an Escherichia coli strain carrying the hrp cluster of Erwinia chrysanthemi caused water-soaking and necrosis. WtsE-induced cell death was not inhibited by cycloheximide treatment, unlike the hypersensitive response caused by a known Avr protein, AvrRxo1. WtsF, the putative chaperone of WtsE, was not required for secretion of WtsE from P. stewartii, and the virulence of wtsF mutants was reduced only at low inoculum concentrations. However, WtsF was required for full accumulation of WtsE within the bacteria at low temperatures. In contrast, WtsF was needed for efficient delivery of WtsE from E. coli via the Erwinia chrysanthemi Hrp system.
Bibliography:	http://dx.doi.org/10.1094/MPMI-19-1092 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0894-0282 1943-7706
DOI:	10.1094/MPMI-19-1092