Survival of P. falciparum infected red blood cell aggregates in elongational shear flow

Rosetting, the formation of red blood cell aggregates, is a life-threatening condition in malaria tropica and not yet fully understood. We study rosette stability using a set of microfluidic stenotic channels, with varied narrowing angle and erythrocytes of blood groups O and A. We find reduced abil...

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Bibliographic Details
Published in:	Lab on a chip Vol. 24; no. 4; p. 787
Main Authors:	Jötten, Anna M, Schepp, Anabelle, Machon, Adam, Moll, Kirsten, Wahlgren, Mats, Krüger, Timm, Westerhausen, Christoph
Format:	Journal Article
Language:	English
Published:	England 13-02-2024
Subjects:	Constriction, Pathologic Erythrocytes Humans Malaria, Falciparum Plasmodium falciparum Protein Binding
Online Access:	Get more information
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Summary:	Rosetting, the formation of red blood cell aggregates, is a life-threatening condition in malaria tropica and not yet fully understood. We study rosette stability using a set of microfluidic stenotic channels, with varied narrowing angle and erythrocytes of blood groups O and A. We find reduced ability of a rosette to pass a stenosis without disruption, the longer the tapered part of the constriction and the narrower the stenosis is. In general, this ability increases with rosette size and is 5-15% higher in blood group A. The experimental results are substantiated by equivalent experiments using lectin-induced red blood cell aggregates and a simulation of the underlying protein binding kinetics.
ISSN:	1473-0189
DOI:	10.1039/d3lc00552f