Search Results - "Machleidt, Thomas"

NanoLuc Complementation Reporter Optimized for Accurate Measurement of Protein Interactions in Cells by Dixon, Andrew S, Schwinn, Marie K, Hall, Mary P, Zimmerman, Kris, Otto, Paul, Lubben, Thomas H, Butler, Braeden L, Binkowski, Brock F, Machleidt, Thomas, Kirkland, Thomas A, Wood, Monika G, Eggers, Christopher T, Encell, Lance P, Wood, Keith V

“…Protein-fragment complementation assays (PCAs) are widely used for investigating protein interactions. However, the fragments used are structurally compromised…”
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NanoBRETA Novel BRET Platform for the Analysis of Protein–Protein Interactions by Machleidt, Thomas, Woodroofe, Carolyn C, Schwinn, Marie K, Méndez, Jacqui, Robers, Matthew B, Zimmerman, Kris, Otto, Paul, Daniels, Danette L, Kirkland, Thomas A, Wood, Keith V

“…Dynamic interactions between proteins comprise a key mechanism for temporal control of cellular function and thus hold promise for development of novel drug…”
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Quantitative, Wide-Spectrum Kinase Profiling in Live Cells for Assessing the Effect of Cellular ATP on Target Engagement by Vasta, James D, Corona, Cesear R, Wilkinson, Jennifer, Zimprich, Chad A, Hartnett, James R, Ingold, Morgan R, Zimmerman, Kristopher, Machleidt, Thomas, Kirkland, Thomas A, Huwiler, Kristin G, Ohana, Rachel Friedman, Slater, Michael, Otto, Paul, Cong, Mei, Wells, Carrow I, Berger, Benedict-Tilman, Hanke, Thomas, Glas, Carina, Ding, Ke, Drewry, David H, Huber, Kilian V M, Willson, Timothy M, Knapp, Stefan, Müller, Susanne, Meisenheimer, Poncho L, Fan, Frank, Wood, Keith V, Robers, Matthew B

“…For kinase inhibitors, intracellular target selectivity is fundamental to pharmacological mechanism. Although a number of acellular techniques have been…”
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Application of BRET to monitor ligand binding to GPCRs by Stoddart, Leigh A, Johnstone, Elizabeth K M, Wheal, Amanda J, Goulding, Joëlle, Robers, Matthew B, Machleidt, Thomas, Wood, Keith V, Hill, Stephen J, Pfleger, Kevin D G

“…Bioluminescence resonance energy transfer, from the substrate of a luciferase fused with a G protein–coupled receptor to a fluorescent dye covalently linked to…”
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Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone Substrate by Hall, Mary P, Unch, James, Binkowski, Brock F, Valley, Michael P, Butler, Braeden L, Wood, Monika G, Otto, Paul, Zimmerman, Kristopher, Vidugiris, Gediminas, Machleidt, Thomas, Robers, Matthew B, Benink, Hélène A, Eggers, Christopher T, Slater, Michael R, Meisenheimer, Poncho L, Klaubert, Dieter H, Fan, Frank, Encell, Lance P, Wood, Keith V

“…Bioluminescence methodologies have been extraordinarily useful due to their high sensitivity, broad dynamic range, and operational simplicity. These…”
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Monitoring phosphorylation and acetylation of CRISPR-mediated HiBiT-tagged endogenous proteins by Alves, Juliano, Schwinn, Marie, Machleidt, Thomas, Goueli, Said A., Cali, James J., Zegzouti, Hicham

“…Intracellular pathways transduce signals through changes in post-translational modifications (PTMs) of effector proteins. Among the approaches used to monitor…”
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Simultaneous inhibition of DNA-PK and Polϴ improves integration efficiency and precision of genome editing by Wimberger, Sandra, Akrap, Nina, Firth, Mike, Brengdahl, Johan, Engberg, Susanna, Schwinn, Marie K., Slater, Michael R., Lundin, Anders, Hsieh, Pei-Pei, Li, Songyuan, Cerboni, Silvia, Sumner, Jonathan, Bestas, Burcu, Schiffthaler, Bastian, Magnusson, Björn, Di Castro, Silvio, Iyer, Preeti, Bohlooly-Y, Mohammad, Machleidt, Thomas, Rees, Steve, Engkvist, Ola, Norris, Tyrell, Cadogan, Elaine B., Forment, Josep V., Šviković, Saša, Akcakaya, Pinar, Taheri-Ghahfarokhi, Amir, Maresca, Marcello

“…Genome editing, specifically CRISPR/Cas9 technology, has revolutionized biomedical research and offers potential cures for genetic diseases. Despite rapid…”
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Exploiting an Asp-Glu "switch" in glycogen synthase kinase 3 to design paralog-selective inhibitors for use in acute myeloid leukemia by Wagner, Florence F, Benajiba, Lina, Campbell, Arthur J, Weïwer, Michel, Sacher, Joshua R, Gale, Jennifer P, Ross, Linda, Puissant, Alexandre, Alexe, Gabriela, Conway, Amy, Back, Morgan, Pikman, Yana, Galinsky, Ilene, DeAngelo, Daniel J, Stone, Richard M, Kaya, Taner, Shi, Xi, Robers, Matthew B, Machleidt, Thomas, Wilkinson, Jennifer, Hermine, Olivier, Kung, Andrew, Stein, Adam J, Lakshminarasimhan, Damodharan, Hemann, Michael T, Scolnick, Edward, Zhang, Yan-Ling, Pan, Jen Q, Stegmaier, Kimberly, Holson, Edward B

“…Glycogen synthase kinase 3 (GSK3), a key regulatory kinase in the wingless-type MMTV integration site family (WNT) pathway, is a therapeutic target of interest…”
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Cell-based, bioluminescent assay for monitoring the interaction between PCSK9 and the LDL receptor by Duellman, Sarah J., Machleidt, Thomas, Cali, James J., Vidugiriene, Jolanta

“…Monitoring the expression of cell-surface receptors, their interaction with extracellular ligands, and their fate upon ligand binding is important for…”
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Identification of a new JNK inhibitor targeting the JNK-JIP interaction site by Stebbins, John L, De, Surya K, Machleidt, Thomas, Becattini, Barbara, Vazquez, Jesus, Kuntzen, Christian, Chen, Li-Hsing, Cellitti, Jason F, Riel-Mehan, Megan, Emdadi, Aras, Solinas, Giovanni, Karin, Michael, Pellecchia, Maurizio

“…JNK is a stress-activated protein kinase that modulates pathways implicated in a variety of disease states. JNK-interacting protein-1 (JIP1) is a scaffolding…”
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An Integrated Approach toward NanoBRET Tracers for Analysis of GPCR Ligand Engagement by Killoran, Michael P., Levin, Sergiy, Boursier, Michelle E., Zimmerman, Kristopher, Hurst, Robin, Hall, Mary P., Machleidt, Thomas, Kirkland, Thomas A., Friedman Ohana, Rachel

“…Gaining insight into the pharmacology of ligand engagement with G-protein coupled receptors (GPCRs) under biologically relevant conditions is vital to both…”
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5,5-Dialkylluciferins are thermal stable substrates for bioluminescence-based detection systems by Shi, Ce, Killoran, Michael P, Hall, Mary P, Otto, Paul, Wood, Monika G, Strauss, Ethan, Encell, Lance P, Machleidt, Thomas, Wood, Keith V, Kirkland, Thomas A

“…Firefly luciferase-based ATP detection assays are frequently used as a sensitive, cost-efficient method for monitoring hygiene in many industrial settings…”
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Luciferase Calibrants Enable Absolute Quantitation of Bioluminescence Power by Klein, Mark A., Lazarev, Sergey, Gervasi, Charles, Cowan, Cristopher, Machleidt, Thomas, Friedman Ohana, Rachel

“…Bioluminescence emitted from a luciferase-catalyzed oxidation of luciferin has been broadly utilized to report on biological events, predominantly through…”
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Development of a rapid, simple, and sensitive point-of-care technology platform utilizing ternary NanoLuc by Torio, Emily A., Ressler, Valerie T., Kincaid, Virginia A., Hurst, Robin, Hall, Mary P., Encell, Lance P., Zimmerman, Kristopher, Forsyth, Stuart K., Rehrauer, William M., Accola, Molly A., Hsu, Chia-Chang, Machleidt, Thomas, Dart, Melanie L.

“…Point-of-care tests are highly valuable in providing fast results for medical decisions for greater flexibility in patient care. Many diagnostic tests, such as…”
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Design, Synthesis, and Structure−Activity Relationship of Substrate Competitive, Selective, and in Vivo Active Triazole and Thiadiazole Inhibitors of the c-Jun N-Terminal Kinase by De, Surya K, Stebbins, John L, Chen, Li-Hsing, Riel-Mehan, Megan, Machleidt, Thomas, Dahl, Russell, Yuan, Hongbin, Emdadi, Aras, Barile, Elisa, Chen, Vida, Murphy, Ria, Pellecchia, Maurizio

“…We report comprehensive structure−activity relationship studies on a novel series of c-Jun N-terminal kinase (JNK) inhibitors. The compounds are substrate…”
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Identification of known drugs targeting the endoplasmic reticulum stress response by Bi, Kun, Nishihara, Kana, Machleidt, Thomas, Hermanson, Spencer, Wang, Jun, Sakamuru, Srilatha, Huang, Ruili, Xia, Menghang

“…The endoplasmic reticulum (ER), a multifunctional organelle, plays a central role in cellular signaling, development, and stress response. Dysregulation of ER…”
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Dual control of caveolar membrane traffic by microtubules and the actin cytoskeleton by Mundy, Dorothy I, Machleidt, Thomas, Ying, Yun-shu, Anderson, Richard G W, Bloom, George S

“…Live cell, time-lapse microscopy was used to study trafficking of caveolin-1-GFP in stably expressing CHO cells. Multiple cytological and biochemical tests…”
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Real-time analysis of the binding of fluorescent VEGF 165 a to VEGFR2 in living cells: Effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor complexes by Kilpatrick, Laura E, Friedman-Ohana, Rachel, Alcobia, Diana C, Riching, Kristin, Peach, Chloe J, Wheal, Amanda J, Briddon, Stephen J, Robers, Matthew B, Zimmerman, Kris, Machleidt, Thomas, Wood, Keith V, Woolard, Jeanette, Hill, Stephen J

“…Vascular endothelial growth factor (VEGF) is an important mediator of angiogenesis. Here we have used a novel stoichiometric protein-labeling method to…”
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Functional dichotomy of neutral and acidic sphingomyelinases in tumor necrosis factor signaling by Wiegmann, K, Schütze, S, Machleidt, T, Witte, D, Krönke, M

“…Ceramide produced by sphingomyelinases (SMases) has been recognized as an important second messenger in growth factor receptor signaling. Tumor necrosis factor…”
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Simian Virus 40 Small Tumor Antigen Induces Deregulation of the Actin Cytoskeleton and Tight Junctions in Kidney Epithelial Cells by Nunbhakdi-Craig, Viyada, Craig, Leonard, Machleidt, Thomas, Sontag, Estelle

“…Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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