Search Results - "Machleidt, I"

Epoxysuccinyl peptide-derived affinity labels for cathepsin B by Schaschke, N, Assfalg-Machleidt, I, Laßleben, Th, Sommerhoff, C.P, Moroder, L, Machleidt, W

“…Extracellular cysteine proteases, in particular cathepsin B, have been implicated in a variety of pathological processes. Selectively targeting labels of this…”
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Rapid enzymatic test for phenotypic HIV protease drug resistance by Hoffmann, Dieter, Assfalg-Machleidt, Irmgard, Nitschko, Hans, von der Helm, Klaus, Koszinowski, Ulrich, Machleidt, Werner

“…A phenotypic resistance test based on recombinant expression of the active HIV protease in E. coli from patient blood samples was developed. The protease is…”
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Cyclodextrins as templates for the presentation of protease inhibitors by Schaschke, N., Musiol, H.-J., Assfalg-Machleidt, I., Machleidt, W., Rudolph-Böhner, S., Moroder, L.

“…Mono(6-succinylamido-6-deoxy)-β-cyclodextrin was synthesized by classical carbohydrate chemistry and used as a template mono-functionalized with the linear,…”
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Cystatins as calpain inhibitors: engineered chicken cystatin- and stefin B-kininogen domain 2 hybrids support a cystatin-like mode of interaction with the catalytic subunit of mu-calpain by Díaz, B G, Gross, S, Assfalg-Machleidt, I, Pfeiler, D, Gollmitzer, N, Gabrijelcic-Geiger, D, Stubbs, M T, Fritz, H, Auerswald, E A, Machleidt, W

“…Within the cystatin superfamily, only kininogen domain 2 (KD2) is able to inhibit mu- and m-calpain. In an attempt to elucidate the structural requirements of…”
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Proteinase-sensitive regions in the heavy chain of low molecular weight kininogen map to the inter-domain junctions by Vogel, R, Assfalg-Machleidt, I, Esterl, A, Machleidt, W, Müller-Esterl, W

“…Low molecular weight kininogen from human plasma was subjected to limited proteolysis with trypsin, chymotrypsin, elastase, and bromelain, and the resulting…”
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Ionomycin-activated calpain triggers apoptosis. A probable role for Bcl-2 family members by Gil-Parrado, Shirley, Fernández-Montalván, Amaury, Assfalg-Machleidt, Irmgard, Popp, Oliver, Bestvater, Felix, Holloschi, Andreas, Knoch, Tobias A, Auerswald, Ennes A, Welsh, Katherine, Reed, John C, Fritz, Hans, Fuentes-Prior, Pablo, Spiess, Eberhard, Salvesen, Guy S, Machleidt, Werner

“…Ubiquitous calpains (mu- and m-calpain) have been repeatedly implicated in apoptosis, but the underlying mechanism(s) remain(s) to be elucidated. We examined…”
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Beef heart complex III: isolation and characterization of cytochrome b by Von Jagow, G., Schägger, H., Engel, W.D., Machleidt, W., Machleidt, I., Kolb, H.J.

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Substrate/propeptide-derived endo-epoxysuccinyl peptides as highly potent and selective cathepsin B inhibitors by Schaschke, Norbert, Assfalg-Machleidt, Irmgard, Machleidt, Werner, Moroder, Luis

“…Based on recent information about the anti-substrate binding mode of the propeptide portion of procathepsin B and the well established substrate-like binding…”
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Inhibition of ATPase, GTPase and adenylate kinase activities of the second nucleotide-binding fold of the cystic fibrosis transmembrane conductance regulator by genistein by Randak, C, Auerswald, E A, Assfalg-Machleidt, I, Reenstra, W W, Machleidt, W

“…In the presence of ATP, genistein, like the ATP analogue adenosine 5'-[beta,gamma-imido]triphosphate (pp[NH]pA), increases cystic fibrosis transmembrane…”
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Calpastatin exon 1B-derived peptide, a selective inhibitor of calpain: enhancing cell permeability by conjugation with penetratin by Gil-Parrado, Shirley, Assfalg-Machleidt, Irmgard, Fiorino, Ferdinando, Deluca, Dominga, Pfeiler, Dietmar, Schaschke, Norbert, Moroder, Luis, Machleidt, Werner

“…The ubiquitous calpains, mu- and m-calpain, have been implicated in essential physiological processes and various pathologies. Cell-permeable specific…”
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A novel type of bifunctional inhibitor directed against proteolytic activity and receptor/ligand interaction. Cystatin with a urokinase receptor binding site by Muehlenweg, B, Assfalg-Machleidt, I, Parrado, S G, Bürgle, M, Creutzburg, S, Schmitt, M, Auerswald, E A, Machleidt, W, Magdolen, V

“…Cancer invasion and metastasis is a process requiring a coordinated series of (anti-)adhesive, migratory, and pericellular proteolytic events involving various…”
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N-terminal variants of recombinant stefin B: effect on affinity for papain and cathepsin B by Thiele, U, Assfalg-Machleidt, I, Machleidt, W, Auerswald, E A

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Enzymatically active cathepsin B dissociating from its inhibitor complexes is elevated in blood plasma of patients with septic shock and some malignant tumors by Assfalg-Machleidt, I, Jochum, M, Klaubert, W, Inthorn, D, Machleidt, W

“…Using fluorogenic substrates and the specific inhibitor E-64, cysteine proteinase (CP) activity was measured in blood plasma of healthy controls (mean = 35.0…”
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β-Cyclodextrin/epoxysuccinyl peptide conjugates : a new drug targeting system for tumor cells by SCHASCHKE, N, ASSFALG-MACHLEIDT, I, MACHLEIDT, W, LASSLEBEN, T, SOMMERHOFF, C. P, MORODER, L

“…Beta-cyclodextrin is known to form inclusion complexes with hydrophobic drugs. Several tumor cell lines are known to secrete and/or contain membrane-associated…”
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E-64 analogues as inhibitors of cathepsin B. On the role of the absolute configuration of the epoxysuccinyl group by Schaschke, Norbert, Assfalg-Machleidt, Irmgard, Machleidt, Werner, Turk, Dushan, Moroder, Luis

“…A series of trans-epoxysuccinyl-peptide derivatives based on the natural inhibitor E-64 were synthesized in the (2 R,3 R) and (2 S,3 S) configuration in order…”
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Recombinant Q53E- and Q53N--chicken egg white cystatin variants inhibit papain, actinidin and cathepsin B by Genenger, G, Lenzen, S, Mentele, R, Assfalg-Machleidt, I, Auerswald, E A

“…A cloned synthetic gene coding for (AEF S1M M29I M89L) chicken egg white cystatin was modified site-specifically at position Q53 by cassette mutagenesis. Two…”
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A recombinant polypeptide model of the second nucleotide-binding fold of the cystic fibrosis transmembrane conductance regulator functions as an active ATPase, GTPase and adenylate kinase by Randak, Christoph, Neth, Peter, Auerswald, Ennes A, Eckerskorn, Christoph, Assfalg-Machleidt, Irmgard, Machleidt, Werner

“…CFTR–NBF-2 expressed and purified in fusion with the maltose-binding protein was shown to catalyse the reaction ATP→ADP+P i by three different assays,…”
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Proteolysis of defensive proteins in peritonitis exudate: pathobiochemical aspects and therapeutical approach by Billing, A, Fröhlich, D, Assfalg-Machleidt, I, Machleidt, W, Jochum, M

“…Peritonitis exudate reveals strong proteolytic activity which is paralleled by deficient opsonic capacity and high concentrations of lysosomal proteinases…”
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Novel bi- and trifunctional inhibitors of tumor-associated proteolytic systems by Krol, Janna, Sato, Sumito, Rettenberger, Peter, Assfalg-Machleidt, Irmgard, Schmitt, Manfred, Magdolen, Viktor, Magdolen, Ulla

“…Serine proteases, cysteine proteases, and matrix metalloproteinases (MMPs) are involved in cancer cell invasion and metastasis. Recently, a recombinant…”
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Cathepsin B-indicator for the release of lysosomal cysteine proteinases in severe trauma and inflammation by Assfalg-Machleidt, I, Jochum, M, Nast-Kolb, D, Siebeck, M, Billing, A, Joka, T, Rothe, G, Valet, G, Zauner, R, Scheuber, H P

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