Search Results - "Macdonald, Patrick J."

Cardiolipin's propensity for phase transition and its reorganization by dynamin-related protein 1 form a basis for mitochondrial membrane fission by Stepanyants, Natalia, Macdonald, Patrick J, Francy, Christopher A, Mears, Jason A, Qi, Xin, Ramachandran, Rajesh

“…Cardiolipin (CL) is an atypical, dimeric phospholipid essential for mitochondrial dynamics in eukaryotic cells. Dynamin-related protein 1 (Drp1), a cytosolic…”
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A dimeric equilibrium intermediate nucleates Drp1 reassembly on mitochondrial membranes for fission by Macdonald, Patrick J, Stepanyants, Natalia, Mehrotra, Niharika, Mears, Jason A, Qi, Xin, Sesaki, Hiromi, Ramachandran, Rajesh

“…The GTPase dynamin-related protein 1 (Drp1) catalyzes mitochondrial division, but the mechanisms remain poorly understood. Much of what is attributed to Drp1's…”
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Distinct Splice Variants of Dynamin-related Protein 1 Differentially Utilize Mitochondrial Fission Factor as an Effector of Cooperative GTPase Activity by Macdonald, Patrick J., Francy, Christopher A., Stepanyants, Natalia, Lehman, Lance, Baglio, Anthony, Mears, Jason A., Qi, Xin, Ramachandran, Rajesh

“…Multiple isoforms of the mitochondrial fission GTPase dynamin-related protein 1 (Drp1) arise from the alternative splicing of its single gene-encoded pre-mRNA…”
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Ester Formation and Hydrolysis during Wet–Dry Cycles: Generation of Far-from-Equilibrium Polymers in a Model Prebiotic Reaction by Mamajanov, Irena, MacDonald, Patrick J, Ying, Jingya, Duncanson, Daniel M, Dowdy, Garrett R, Walker, Chelsea A, Engelhart, Aaron E, Fernández, Facundo M, Grover, Martha A, Hud, Nicholas V, Schork, F. Joseph

“…Biopolymers exist within living cells as far-from-equilibrium metastable polymers. Living systems must constantly invest energy for biopolymer synthesis. In…”
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Chromophore maturation and fluorescence fluctuation spectroscopy of fluorescent proteins in a cell-free expression system by Macdonald, Patrick J., Chen, Yan, Mueller, Joachim D.

“…Cell-free synthesis, a method for the rapid expression of proteins, is increasingly used to study interactions of complex biological systems. GFP and its…”
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Quantitative measurement of brightness from living cells in the presence of photodepletion by Hur, Kwang-Ho, Macdonald, Patrick J, Berk, Serkan, Angert, C Isaac, Chen, Yan, Mueller, Joachim D

“…The brightness of fluorescently labeled proteins provides an excellent marker for identifying protein interactions in living cells. Quantitative interpretation…”
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Quantifying Protein-Protein Interactions of Peripheral Membrane Proteins by Fluorescence Brightness Analysis by Smith, Elizabeth M., Macdonald, Patrick J., Chen, Yan, Mueller, Joachim D.

“…Fluorescently labeled proteins that are found both in the cytoplasm and at the plasma membrane, such as peripheral membrane proteins, create stratified…”
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Affinity of anti-spike antibodies in SARS-CoV-2 patient plasma and its effect on COVID-19 antibody assays by Macdonald, Patrick J., Ruan, Qiaoqiao, Grieshaber, Jessica L., Swift, Kerry M., Taylor, Russell E., Prostko, John C., Tetin, Sergey Y.

“…Measuring anti-spike protein antibodies in human plasma or serum is commonly used to determine prior exposure to SARS-CoV-2 infection and to assess the…”
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Affinity of anti-spike antibodies to three major SARS-CoV-2 variants in recipients of three major vaccines by Macdonald, Patrick J., Schaub, Jeffrey M., Ruan, Qiaoqiao, Williams, Carroll L., Prostko, John C., Tetin, Sergey Y.

“…Background Measuring anti-viral antibody affinity in blood plasma or serum is a rational quantitative approach to assess humoral immune response and acquired…”
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Characterization of Cytoplasmic Gag-Gag Interactions by Dual-Color Z-Scan Fluorescence Fluctuation Spectroscopy by Fogarty, Keir H., Chen, Yan, Grigsby, Iwen F., Macdonald, Patrick J., Smith, Elizabeth M., Johnson, Jolene L., Rawson, Jonathan M., Mansky, Louis M., Mueller, Joachim D.

“…Fluorescence fluctuation spectroscopy (FFS) quantifies the interactions of fluorescently-labeled proteins inside living cells by brightness analysis. However,…”
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Direct single-molecule imaging for diagnostic and blood screening assays by Ruan, Qiaoqiao, Macdonald, Patrick J., Swift, Kerry M., Tetin, Sergey Y.

“…Every year, over 100 million units of donated blood undergo mandatory screening for HIV, hepatitis B, hepatitis C, and syphilis worldwide. Often, donated blood…”
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Method for direct measuring antibody affinity in biological sample by Ruan, Qiaoqiao, Schaub, Jeffrey M., Macdonald, Patrick J., Tetin, Sergey Y.

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Rhodamine-Derived Fluorescent Dye with Inherent Blinking Behavior for Super-Resolution Imaging by Macdonald, Patrick J, Gayda, Susan, Haack, Richard A, Ruan, Qiaoqiao, Himmelsbach, Richard J, Tetin, Sergey Y

“…Super-resolution microscopy enables imaging of structures smaller than the diffraction limit. Single-molecule localization microscopy methods, such as…”
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Direct single-molecule counting for immunoassay applications by Macdonald, Patrick J., Ruan, Qiaoqiao, Tetin, Sergey Y.

“…Single-molecule methods offer specificity in studying complex systems and dynamics, but they also offer high sensitivity for basic enumeration. We apply…”
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Single-cell clinical chemistry: Exploring glucose uptake kinetics and HbA1c levels in individual red blood cells by Macdonald, Patrick J., Paprocki, Joel D., Xu, Yongjin, Cheng, Alan, Dunn, Tim C., Tetin, Sergey Y.

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A Single Common Protocol for the Expression and Purification of Soluble Mammalian DSPs from Escherichia coli by Stepanyants, Natalia, Macdonald, Patrick J, Madan Mohan, Pooja, Ramachandran, Rajesh

“…Mammalian DSPs have been historically isolated either from native tissue sources or from transfected insect cell cultures via time-consuming and cumbersome…”
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Steric interference from intrinsically disordered regions controls dynamin-related protein 1 self-assembly during mitochondrial fission by Lu, Bin, Kennedy, Bridget, Clinton, Ryan W., Wang, Emily Jue, McHugh, Daniel, Stepanyants, Natalia, Macdonald, Patrick J., Mears, Jason A., Qi, Xin, Ramachandran, Rajesh

“…The self-assembling, mechanoenzymatic dynamin superfamily GTPase, dynamin-related protein 1 (Drp1), catalyzes mitochondrial and peroxisomal fission. Distinct…”
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APOBEC3 Multimerization Correlates with HIV-1 Packaging and Restriction Activity in Living Cells by Li, Jinhui, Chen, Yan, Li, Ming, Carpenter, Michael A., McDougle, Rebecca M., Luengas, Elizabeth M., Macdonald, Patrick J., Harris, Reuben S., Mueller, Joachim D.

“…APOBEC3G belongs to a family of DNA cytosine deaminases that are involved in the restriction of a broad number of retroviruses including human immunodeficiency…”
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Brightness Analysis by Z-Scan Fluorescence Fluctuation Spectroscopy for the Study of Protein Interactions within Living Cells by Macdonald, Patrick J., Chen, Yun, Wang, Xiao, Chen, Yan, Mueller, Joachim D.

“…Fluorescence fluctuation spectroscopy (FFS) quantifies interactions of fluorescently labeled proteins inside living cells by brightness analysis. Conventional…”
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Probing nucleocytoplasmic transport by two-photon activation of PA-GFP by Chen, Yan, Macdonald, Patrick J., Skinner, Joseph P., Patterson, George H., Müller, Joachim D.

“…Two‐photon activation of photoactivatable green fluorescent protein (PA‐GFP) provides a unique tool for probing cellular transport processes, because…”
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