Triphasic production of IFN γ by innate and adaptive lymphocytes following influenza A virus infection
Interferon gamma (IFN ) is a potent antiviral cytokine that can be produced by many innate and adaptive immune cells during infection. Currently, our understanding of which cells produce IFN and where they are located at different stages of an infection is limited. We have used reporter mice to inve...
Saved in:
| Published in: | Discovery immunology Vol. 2; no. 1; p. kyad014 |
|---|---|
| Main Authors: | , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
England
Oxford University Press
2023
|
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Interferon gamma (IFN
) is a potent antiviral cytokine that can be produced by many innate and adaptive immune cells during infection. Currently, our understanding of which cells produce IFN
and where they are located at different stages of an infection is limited. We have used reporter mice to investigate
expression of
mRNA in the lung and secondary lymphoid organs during and following influenza A virus (IAV) infection. We observed a triphasic production of
expression. Unconventional T cells and innate lymphoid cells, particularly NK cells, were the dominant producers of early
, while CD4 and CD8 T cells were the main producers by day 10 post-infection. Following viral clearance, some memory CD4 and CD8 T cells continued to express
in the lungs and draining lymph node. Interestingly,
production by lymph node natural killer (NK), NKT, and innate lymphoid type 1 cells also continued to be above naïve levels, suggesting memory-like phenotypes for these cells. Analysis of the localization of
+ memory CD4 and CD8 T cells demonstrated that cytokine+ T cells were located near airways and in the lung parenchyma. Following a second IAV challenge, lung IAV-specific CD8 T cells rapidly increased their expression of
while CD4 T cells in the draining lymph node increased their
response. Together, these data suggest that
production fluctuates based on cellular source and location, both of which could impact subsequent immune responses. |
|---|---|
| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present address: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, UK Present address: Institute of Immunity and Transplantation, University College London, London, UK |
| ISSN: | 2754-2483 2754-2483 |
| DOI: | 10.1093/discim/kyad014 |