The Effect of Encapsulated Apigenin Nanoparticles on HePG-2 Cells through Regulation of P53

The Effect of Encapsulated Apigenin Nanoparticles on HePG-2 Cells through Regulation of P53

Apigenin (Ap) is one of the most important natural flavonoids that has potent anticancer activity. This study was designed, for the first time, to load Ap into chitosan to improve its hydrophobicity and then it was coated with albumin-folic acid to increase its stability and bioavailability and to t...

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Bibliographic Details
Published in:Pharmaceutics Vol. 14; no. 6; p. 1160
Main Authors: Mabrouk Zayed, Mayada Mohamed, Sahyon, Heba A., Hanafy, Nemany A. N., El-Kemary, Maged A.
Format: Journal Article
Language:English
Published: Basel MDPI AG 29-05-2022
MDPI
Subjects:
Acids
apigenin
Apoptosis
Bioavailability
Cancer therapies
capsase
Cell cycle
Cytotoxicity
Drugs
Efficiency
Flavonoids
Fourier transforms
hepatocellular carcinoma
Liver cancer
Nanoparticles
Physiology
Polyphenols
Radiation
Spectrum analysis
Vitamin B
Japan
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Summary:Apigenin (Ap) is one of the most important natural flavonoids that has potent anticancer activity. This study was designed, for the first time, to load Ap into chitosan to improve its hydrophobicity and then it was coated with albumin-folic acid to increase its stability and bioavailability and to target cancer cells. The newly developed encapsulated Ap (Ap-CH-BSA-FANPs) was characterized and tested in vitro. The zeta potential of −17.0 mV was within the recommended range (−30 mV to +30 mV), indicating that encapsulated apigenin would not quickly settle and would be suspended. The in vitro results proved the great anticancer activity of the encapsulated apigenin on HePG-2 cells compared to pure Ap. The treated HePG-2 cells with Ap-CH-BSA-FANPs demonstrated the induction of apoptosis by increasing p53 gene expression, arresting the cell cycle, increasing caspase-9 levels, and decreasing both the MMP9 gene and Bcl-2 protein expression levels. Moreover, the higher antioxidant activity of the encapsulated apigenin treatment was evident through increasing SOD levels and decreasing the CAT concentration. In conclusion, the Ap-CH-BSA-FANPs were easy to produce with low coast, continued drug release, good loading capacity, high solubility in physiological pH, and were more stable than the formerly Ap-loaded liposomes or PLGA. Moreover, Ap-CH-BSA-FANPs may be a promising chemotherapeutic agent in the treatment of HCC.
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These authors contributed equally to this work.
ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics14061160