Surface free energy and bacterial retention to saliva-coated dental implant materials—an in vitro study

The aim of the present investigation was to compare the in vitro bacterial retention on saliva-coated implant materials (pure titanium grade 2 (cp-Ti) and a titanium alloy (Ti–6Al–4V) surfaces), presenting similar surface roughness, and to assess the influence of physico-chemical surface properties...

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Bibliographic Details
Published in:	Colloids and surfaces, B, Biointerfaces Vol. 39; no. 4; pp. 199 - 205
Main Authors:	Mabboux, Florence, Ponsonnet, Laurence, Morrier, Jean-Jacques, Jaffrezic, Nicole, Barsotti, Odile
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 25-12-2004 Elsevier
Subjects:	Bacterial Adhesion - physiology Bacterial retention Dental Alloys - chemistry Dental implants Dental Implants - microbiology Dental Materials - chemistry Electric power Energy Metabolism Engineering Sciences Entropy Hydrophobic and Hydrophilic Interactions Hydrophobicity Oral streptococci Saliva - chemistry Saliva - microbiology Streptococcus constellatus - metabolism Streptococcus sanguis - metabolism Surface Properties Titanium - chemistry Surface properties Hydrophobicity Dental implants Bacterial retention Oral streptococci bacterial retention DENTURE BASE MATERIALS oral streptococci WETTABILITY PELLICLE MICROBIAL ADHESION COLONIZATION TITANIUM HYDROXYAPATITE dental implants STREPTOCOCCI ADHERENCE hydrophobicity PLAQUE-FORMATION surface properties
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Summary:	The aim of the present investigation was to compare the in vitro bacterial retention on saliva-coated implant materials (pure titanium grade 2 (cp-Ti) and a titanium alloy (Ti–6Al–4V) surfaces), presenting similar surface roughness, and to assess the influence of physico-chemical surface properties of bacterial strain and implant materials on in vitro bacterial adherence. Two bacterial strains (one hydrophilic strain and one hydrophobic strain) were used and the following were evaluated: bacterial cell adherence, SFE values as well as the Lifshitz-van-der Waals, the Lewis acid base components of SFE, the interfacial free energy and the non-dispersive interactions according to two complementary contact angle measurement methods: the sessile drop method and the captive bubble method. Our results showed similar patterns of adherent bacterial cells on saliva-coated cp-Ti and saliva-coated Ti–6Al–4V. These findings could suggest that bacterial colonization (i.e. plaque formation) is similar on saliva-coated cp-Ti and Ti–6Al–4V surfaces and indicate that both materials could be suitable for use as transgingival abutment or healing implant components. The same physico-chemical properties exhibited by saliva-coated cp-Ti and TA6V, as shown by the sessile drop method and the captive bubble method, could explain this similar bacterial colonisation. Therefore, higher values of total surface free energy of saliva-coated cp-Ti and saliva-coated TA6V samples ( γ SV ≈65 mJ/m 2) were reported using the captive bubble method indicating a less hydrophobic character of these surfaces than with the sessile drop method ( γ S ≈44.50 mJ/m 2) and consequently possible differences in oral bacterial retention according the theory described by Absolom et al. The number of adherent hydrophobic S. sanguinis cells was two-fold higher than that of hydrophilic S. constellatus cells. Our results confirm that physico-chemical surface properties of oral bacterial strains play a role in bacterial retention to implant materials in the presence of adsorbed salivary proteins.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0927-7765 1873-4367
DOI:	10.1016/j.colsurfb.2004.08.002